Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, Calu-1 cells, U-251 cells, U-87 MG cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32267-1-AP targets LINGO3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37289 Product name: Recombinant human LINGO3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 360-530 aa of NM_001101391 Sequence: LLWIVQRRKTLNFDGRLPACATPAEVRGDALRNLPDSVLFEYFVCRKPKIRERRLQRVTATAGEDVRFLCRAEGEPAPTVAWVTPQHRPVTATSAGRARVLPGGTLEIQDARPQDSGTYTCVASNAGGNDTYFATLTVRPEPAANRTPGEAHNETLAALRAPLDLTTILVS 相同性解析による交差性が予測される生物種 |
| Full Name | leucine rich repeat and Ig domain containing 3 |
| Calculated molecular weight | 64kDa |
| Observed molecular weight | 67 kDa |
| GenBank accession number | NM_001101391 |
| Gene Symbol | LINGO3 |
| Gene ID (NCBI) | 645191 |
| RRID | AB_3742558 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P0C6S8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LINGO3 represents a previously unrecognized factor that promotes mucosal wound healing. It partially transduces the repair signals of TFF2, especially through the MAPK signaling cascade, and facilitates epithelial regeneration by preserving the intestinal stem cell microenvironment and maintaining Lgr5 expression (PMID: 33941035).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LINGO3 antibody 32267-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |