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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LIN37 Polyclonal antibody (26651-1-AP)

    WB analysis using 26651-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26651-1-AP (LIN37 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using LIN37 Polyclonal antibody (26651-1-AP)

    IF Staining of A549 using 26651-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using LIN37 antibody (26651-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

LIN37 Polyclonal antibody

LIN37 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26651-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Protein lin-37 homolog, Protein lin 37 homolog, MSTP064, lin 37, F25965

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, Jurkat cells
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26651-1-AP targets LIN37 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIN37 fusion protein Ag24565 相同性解析による交差性が予測される生物種
Full Name lin-37 homolog (C. elegans)
Observed molecular weight 36 kDa
GenBank accession numberBC009071
Gene Symbol LIN37
Gene ID (NCBI) 55957
RRIDAB_2880590
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96GY3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LIN37, also named as Antolefinin and MSTP064, is a component of the DREAM complex (also named LINC complex). LIN37 migrates to 37 kDa for modification.

Protocols

Product Specific Protocols
WB protocol for LIN37 antibody 26651-1-APDownload protocol
IF protocol for LIN37 antibody 26651-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Int J Oncol

Transcription factor E2F4 facilitates SUMOylation to promote HCC progression through interaction with LIN9

Authors - Zhenwei Ma
View Article