LIN28B Polyclonal antibody

LIN28B Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, PLA, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24017-1-AP

Synonyms

CSDD2, lin 28 homolog B (C. elegans), Lin 28B, LIN28B, Protein lin 28 homolog B



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IF detected inNCCIT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

24017-1-AP targets LIN28B in WB, IP, IF, PLA, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIN28B fusion protein Ag21241 相同性解析による交差性が予測される生物種
Full Name lin-28 homolog B (C. elegans)
Calculated molecular weight 250 aa, 27 kDa
Observed molecular weight35 kDa
GenBank accession numberBC137526
Gene symbol LIN28B
Gene ID (NCBI) 389421
RRIDAB_2879403
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LIN28B, also named as CSDD2, belongs to the lin-28 family. It acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7). LIN2B is specifically expressed in embryonic muscle, neurons, and epithelia, as well as in embryonic carcinoma cells. However, it is suppressed in most adult tissues except for skeletal and cardiac muscle. The calculated molecular weight of LIN28B is 27 kDa, but a 35 kDa band has been detected in some articles (PMID: 33029224, 33378003).

Protocols

Product Specific Protocols
WB protocol for LIN28B antibody 24017-1-APDownload protocol
IF protocol for LIN28B antibody 24017-1-APDownload protocol
IP protocol for LIN28B antibody 24017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Front Immunol

Lin28 regulates thymic growth and involution and correlates with MHCII expression in thymic epithelial cells

Authors - Shiyun Xiao
humanWB,PLA

iScience

Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells

Authors - Nam-Kyung Yu
mouseWB

Pharm Biol

Peptides from the croceine croaker (Larimichthys crocea) swim bladder attenuate busulfan-induced oligoasthenospermia in mice.

Authors - Xi Zhao
humanIP, IHC

Int J Clin Exp Pathol

Lin28B is a novel prognostic marker in gastric adenocarcinoma.

Authors - Qian Hu