Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 11724-1-AP
Various lysate were subjected to SDS PAGE followed by western blot with 11724-1-AP (LIN28 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of NCCIT cells using 11724-1-AP
WB data of LIN28 antibody (11724-1-AP, 1:500) with si-control and si-LIN28 transfected NCCIT cells.
IP experiment of K-562 using 11724-1-AP
IP result of anti-LIN28 (IP:11724-1-AP, 4ug; Detection:11724-1-AP 1:2000) with K-562 cells lysate 1360 ug.
IHC staining of mouse testis using 11724-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11724-1-AP (LIN28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 11724-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11724-1-AP (LIN28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human embronic stem cells using 11724-1-AP
Confocal immunofluorescent analysis of human embronic stem cells with 11724-1-AP at dilution of 1:200. The PE shows staining with 11724-1-AP/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI. (10X).
IF Staining of human embronic stem cells using 11724-1-AP
Confocal immunofluorescent analysis of human embronic stem cells with 11724-1-AP at dilution of 1:400. The PE shows staining with 11724-1-AP/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI. (20X).

Featured Product
KD/KO Validated

LIN28A Polyclonal antibody

LIN28A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11724-1-AP

Publications(57)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

Protein lin 28 homolog A, Lin-28A, LIN28, Lin 28A, LIN 28

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (57)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	K-562 cells, NCCIT cells, mouse embryo tissue
Positive IP detected in	K-562 cells
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	human embronic stem cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 29 publications below
IHC	See 18 publications below
IF	See 14 publications below
IP	See 2 publications below
RIP	See 1 publications below

Product Information

11724-1-AP targets LIN28A in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2312 Product name: Recombinant human LIN28 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-209 aa of BC028566 Sequence: MGSVSNQQFAGGCAKAAEEAPEEAPEDAARAADEPQLLHGAGICKWFNVRMGFGFLSMTARAGVALDPPVDVFVHQSKLHMEGFRSLKEGEAVEFTFKKSAKGLESIRVTGPGGVFCIGSERRPKGKSMQKRRSKGDRCYNCGGLDHHAKECKLPPQPKKCHFCQSISHMVASCPLKAQQGPSAQGKPTYFREEEEEIHSPTLLPEAQN 相同性解析による交差性が予測される生物種
Full Name	lin-28 homolog (C. elegans)
Calculated molecular weight	209 aa, 23 kDa
Observed molecular weight	28 kDa
GenBank accession number	BC028566
Gene Symbol	LIN28
Gene ID (NCBI)	79727
RRID	AB_2135039
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H9Z2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell[PMID: 20139967]. LIN28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic mRNA-binding protein that binds to and enhances the translation of the IGF2 mRNA[PMID: 21057460]. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells[PMID: 22078496]. Affinity purified rabbit anti-LIN28 can be used to demonstrate pluripotency of ES and Ips cells, and to detect LIN28 transgene expression in the process of reprogramming. This antibody is a rabbit polyclonal antibody raised against full length LIN28 of human origin. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.

Protocols

Product Specific Protocols
IHC protocol for LIN28A antibody 11724-1-AP	Download protocol
IP protocol for LIN28A antibody 11724-1-AP	Download protocol
WB protocol for LIN28A antibody 11724-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Nature A role for Lin28 in primordial germ-cell development and germ-cell malignancy. Authors - West Jason A JA View Article
human,mouse	WB,IHC	Nat Genet Lin28 promotes transformation and is associated with advanced human malignancies. Authors - Viswanathan Srinivas R SR View Article
mouse	IHC	Nat Genet Lin28a transgenic mice manifest size and puberty phenotypes identified in human genetic association studies. Authors - Zhu Hao H View Article
human	WB,IF	Nat Methods A nonviral minicircle vector for deriving human iPS cells. Authors - Jia Fangjun F View Article
human	IF	Cell Stem Cell Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders. Authors - Carmen Lorenz View Article
mouse	IHC	Cell Res Ythdc2 is an N(6)-methyladenosine binding protein that regulates mammalian spermatogenesis. Authors - Phillip J Hsu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11724-1-AP

WB analysis of NCCIT cells using 11724-1-AP

IP experiment of K-562 using 11724-1-AP

IHC staining of mouse testis using 11724-1-AP

IHC staining of mouse testis using 11724-1-AP

IF Staining of human embronic stem cells using 11724-1-AP

IF Staining of human embronic stem cells using 11724-1-AP

LIN28A Polyclonal antibody

LIN28A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A role for Lin28 in primordial germ-cell development and germ-cell malignancy.

Lin28 promotes transformation and is associated with advanced human malignancies.

Lin28a transgenic mice manifest size and puberty phenotypes identified in human genetic association studies.

A nonviral minicircle vector for deriving human iPS cells.

Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders.

Ythdc2 is an N(6)-methyladenosine binding protein that regulates mammalian spermatogenesis.