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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LIMS2 Polyclonal antibody (55300-1-AP)

    WB analysis using 55300-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55300-1-AP (LIMS2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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LIMS2 Polyclonal antibody

LIMS2 Polyclonal Antibody for WB, ELISA
Cat No. 55300-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

LIM like protein 2, PINCH2, PINCH-2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue, mouse bladder tissue, rat bladder tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55300-1-AP targets LIMS2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name LIM and senescent cell antigen-like domains 2
Calculated molecular weight 39 kDa
Observed molecular weight35-40 kDa
GenBank accession numberNM_017980
Gene Symbol LIMS2
Gene ID (NCBI) 55679
RRIDAB_3670247
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z4I7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LIMS2, also known as PINCH2, is a member of a small family of focal adhesion proteins. LIMS2 contains five LIM domains, each domain forming two zinc fingers, which permit interactions that regulate cell shape and migration. Along with integrin-linked kinase and parvin, LIMS2 is a component of a complex that mediates multiple protein-protein interactions at adhesion sites between cells and the extracellular matrix (ECM) (PMID: 12167643). Mutations in LIMS2 are associated with a novel muscular dystrophy, severe cardiomyopathy and triangular tongues(PMID: 25589244).

Protocols

Product Specific Protocols
WB protocol for LIMS2 antibody 55300-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols