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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LIMS1 Recombinant monoclonal antibody (83368-3-RR)

    WB analysis using 83368-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83368-3-RR (LIMS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using LIMS1 Recombinant monoclonal antibody (83368-3-RR)

    IP experiment of HeLa using 83368-3-RR

    IP result of anti-LIMS1 (IP:83368-3-RR, 4ug; Detection:83368-3-RR 1:1000) with HeLa cells lysate 1000 ug.

LIMS1 Recombinant monoclonal antibody

LIMS1 Uni-rAb® Recombinant Antibody for WB, IP, ELISA
Cat No. 83368-3-RR
Clone No.260013D6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

LIM and senescent cell antigen-like-containing domain protein 1, Particularly interesting new Cys-His protein 1, PINCH, PINCH 1, PINCH1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, A549 cells, Caco-2 cells, K-562 cells, Jurkat cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83368-3-RR targets LIMS1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name LIM and senescent cell antigen-like domains 1
Calculated molecular weight 37 kDa
Observed molecular weight 35 kDa
GenBank accession numberNM_004987
Gene Symbol LIMS1
Gene ID (NCBI) 3987
RRIDAB_3743477
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP48059
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LIMS1, also known as PINCH1, is an adaptor protein that contains five LIM domains, or double zinc fingers(PMID: 7517666). PINCH-1 is a widely expressed focal adhesion protein that forms a ternary complex with integrin-linked kinase (ILK) and CH-ILKBP/actopaxin/alpha-parvin (PMID: 14551191). LIMS1 is involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques.

Protocols

Product Specific Protocols
IP protocol for LIMS1 antibody 83368-3-RRDownload protocol
WB protocol for LIMS1 antibody 83368-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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