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Validation Data Gallery

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  • Western Blot (WB) analysis of U-937 cells using LIMD2 Polyclonal antibody (15471-1-AP)

    WB analysis of U-937 using 15471-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 15471-1-AP (LIMD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using LIMD2 Polyclonal antibody (15471-1-AP)

    WB analysis of Raji using 15471-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 15471-1-AP (LIMD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LIMD2 Polyclonal antibody (15471-1-AP)

    IHC staining of human liver cancer using 15471-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15471-1-AP (LIMD2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LIMD2 Polyclonal antibody (15471-1-AP)

    IHC staining of human liver cancer using 15471-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15471-1-AP (LIMD2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using LIMD2 Polyclonal antibody (15471-1-AP)

    IHC staining of human brain using 15471-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15471-1-AP (LIMD2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using LIMD2 Polyclonal antibody (15471-1-AP)

    IHC staining of human brain using 15471-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15471-1-AP (LIMD2 antibody) at dilution of 1:50 (under 40x lens).

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  • Featured Product
  • KD/KO Validated

LIMD2 Polyclonal antibody

LIMD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15471-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

LIM domain containing 2, LIMD2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU-937 cells, Raji cells
Positive IHC detected inhuman liver cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15471-1-AP targets LIMD2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIMD2 fusion protein Ag7775 相同性解析による交差性が予測される生物種
Full Name LIM domain containing 2
Calculated molecular weight 14 kDa
Observed molecular weight 16-20 kDa
GenBank accession numberBC004400
Gene Symbol LIMD2
Gene ID (NCBI) 80774
RRIDAB_10859817
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BT23
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LIMD2 antibody 15471-1-APDownload protocol
IHC protocol for LIMD2 antibody 15471-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Bioengineered

LIM domain-containing 2 (LIMD2) promotes the progress of ovarian cancer via the focal adhesion signaling pathway.

Authors - Lixin Chen
View Article
humanWB

Mol Biol Rep

Knockdown of LIMD2 inhibits the progression of ovarian carcinoma through ERK1/2 pathway

Authors - Haiyang Hu
  • KD Validated
View Article