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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using LIG4 Polyclonal antibody (12695-1-AP)

    WB analysis of mouse testis using 12695-1-AP (same clone as 12695-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using LIG4 Polyclonal antibody (12695-1-AP)

    WB analysis of HepG2 using 12695-1-AP (same clone as 12695-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using LIG4 Polyclonal antibody (12695-1-AP)

    WB analysis of mouse liver using 12695-1-AP (same clone as 12695-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using LIG4 Polyclonal antibody (12695-1-AP)

    WB analysis of HeLa using 12695-1-AP (same clone as 12695-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using LIG4 Polyclonal antibody (12695-1-AP)

    WB analysis of rat testis using 12695-1-AP (same clone as 12695-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using LIG4 Polyclonal antibody (12695-1-AP)

    IHC staining of human prostate cancer using 12695-1-AP (same clone as 12695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12695-1-AP (LIG4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using LIG4 Polyclonal antibody (12695-1-AP)

    IHC staining of human testis using 12695-1-AP (same clone as 12695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 12695-1-AP (LIG4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using LIG4 Polyclonal antibody (12695-1-AP)

    IHC staining of human prostate cancer using 12695-1-AP (same clone as 12695-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 12695-1-AP (LIG4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

LIG4 Polyclonal antibody, PBS Only

LIG4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12695-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12695-1-PBS targets LIG4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3385

Product name: Recombinant human LIG4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 606-911 aa of BC037491

Sequence: ASGKLASKHLYIGGDDEPQEKKRKAAPKMKKVIGIIEHLKAPNLTNVNKISNIFEDVEFCVMSGTDSQPKPDLENRIAEFGGYIVQNPGPDTYCVIAGSENIRVKNIILSNKHDVVKPAWLLECFKTKSFVPWQPRFMIHMCPSTKEHFAREYDCYGDSYFIDTDLNQLKEVFSGIKNSNEQTPEEMASLIADLEYRYSWDCSPLSMFRRHTVYLDSYAVINDLSTKNEGTRLAIKALELRFHGAKVVSCLAEGVSHVIIGEDHSRVADFKAFRRTFKRKFKILKESWVTDSIDKCELQEENQYLI

相同性解析による交差性が予測される生物種
Full Name ligase IV, DNA, ATP-dependent
Calculated molecular weight 911 aa, 104 kDa
Observed molecular weight 100-104 kDa
GenBank accession numberBC037491
Gene Symbol LIG4
Gene ID (NCBI) 3981
RRIDAB_2136253
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP49917
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand brdownloadeaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.