• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using LIF Polyclonal antibody (26757-1-AP)

    WB analysis of Jurkat using 26757-1-AP (same clone as 26757-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26757-1-AP (LIF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using LIF Polyclonal antibody (26757-1-AP)

    WB analysis of MCF-7 using 26757-1-AP (same clone as 26757-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26757-1-AP (LIF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LIF Polyclonal antibody (26757-1-AP)

    IHC staining of human lung cancer using 26757-1-AP (same clone as 26757-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26757-1-AP (LIF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LIF Polyclonal antibody (26757-1-AP)

    IHC staining of human lung cancer using 26757-1-AP (same clone as 26757-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26757-1-AP (LIF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using LIF Polyclonal antibody (26757-1-AP)

    IHC staining of mouse embryo using 26757-1-AP (same clone as 26757-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 26757-1-AP (LIF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using LIF Polyclonal antibody (26757-1-AP)

    IHC staining of mouse embryo using 26757-1-AP (same clone as 26757-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 26757-1-AP (LIF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse embryo tissue using LIF Polyclonal antibody (26757-1-AP)

    IF Staining of mouse embryo using 26757-1-AP (same clone as 26757-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using LIF antibody (26757-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using LIF Polyclonal antibody (26757-1-AP)

    IF Staining of A431 using 26757-1-AP (same clone as 26757-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using LIF antibody (26757-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

LIF Polyclonal antibody, PBS Only

LIF Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 26757-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

Leukemia inhibitory factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26757-1-PBS targets LIF in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIF fusion protein Ag25076 相同性解析による交差性が予測される生物種
Full Name leukemia inhibitory factor (cholinergic differentiation factor)
Calculated molecular weight 202 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC093733
Gene Symbol LIF
Gene ID (NCBI) 3976
RRIDAB_2880624
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15018
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.