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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using LGI3 Polyclonal antibody (21919-1-AP)

    WB analysis of HepG2 using 21919-1-AP (same clone as 21919-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21919-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LGI3 Polyclonal antibody (21919-1-AP)

    WB analysis using 21919-1-AP (same clone as 21919-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21919-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using LGI3 Polyclonal antibody (21919-1-AP)

    WB analysis of mouse lung using 21919-1-AP (same clone as 21919-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21919-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using LGI3 Polyclonal antibody (21919-1-AP)

    WB analysis of A549 using 21919-1-AP (same clone as 21919-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21919-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using LGI3 Polyclonal antibody (21919-1-AP)

    WB analysis of mouse liver using 21919-1-AP (same clone as 21919-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21919-1-AP (LGI3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21919-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using LGI3 Polyclonal antibody (21919-1-AP)

    IHC staining of human lung using 21919-1-AP (same clone as 21919-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21919-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using LGI3 Polyclonal antibody (21919-1-AP)

    IHC staining of human lung using 21919-1-AP (same clone as 21919-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21919-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using LGI3 Polyclonal antibody (21919-1-AP)

    IHC staining of human lung using 21919-1-AP (same clone as 21919-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21919-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using LGI3 Polyclonal antibody (21919-1-AP)

    IHC staining of human lung using 21919-1-AP (same clone as 21919-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21919-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using LGI3 Polyclonal antibody (21919-1-AP)

    IHC staining of human brain using 21919-1-AP (same clone as 21919-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21919-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using LGI3 Polyclonal antibody (21919-1-AP)

    IHC staining of human brain using 21919-1-AP (same clone as 21919-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 21919-1-AP (LGI3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21919-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LGI3 Polyclonal antibody (21919-1-AP)

    IF Staining of HepG2 using 21919-1-AP (same clone as 21919-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 21919-1-AP (LGI3 Antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-Rabbit IgG (red). This data was developed using the same antibody clone with 21919-1-PBS in a different storage buffer formulation.

LGI3 Polyclonal antibody, PBS Only

LGI3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21919-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21919-1-PBS targets LGI3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16257

Product name: Recombinant human LGI3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 343-524 aa of BC142997

Sequence: HQALHPWHRDTDLEFVDGEGKPRLIVSSSSQAPVIYQWSRTQKQFVAQGEVTQVPDAQAVKHFRAGRDSYLCLSRYIGDSKILRWEGTRFSEVQALPSRGSLALQPFLVGGRRYLALGSDFSFTQIYQWDEGRQKFVRFQELAVQAPRAFCYMPAGDAQLLLAPSFKGQTLVYRHIVVDLSA

相同性解析による交差性が予測される生物種
Full Name leucine-rich repeat LGI family, member 3
Calculated molecular weight 548 aa, 62 kDa
Observed molecular weight 55-62 kDa
GenBank accession numberBC142997
Gene Symbol LGI3
Gene ID (NCBI) 203190
RRIDAB_10838807
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N145
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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