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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LGALS9/Galectin-9 Polyclonal antibody (30409-1-AP)

    WB analysis using 30409-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30409-1-AP (LGALS9/Galectin-9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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LGALS9/Galectin-9 Polyclonal antibody

LGALS9/Galectin-9 Polyclonal Antibody for WB, ELISA
Cat No. 30409-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Galectin 9, LGALS9, Ecalectin, Gal 9, Gal-9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTHP-1 cells, U-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30409-1-AP targets LGALS9/Galectin-9 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LGALS9/Galectin-9 fusion protein Eg0084 相同性解析による交差性が予測される生物種
Full Name lectin, galactoside-binding, soluble, 9
Calculated molecular weight 323 aa, 36 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC110340
Gene Symbol Galectin-9
Gene ID (NCBI) 3965
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00182
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The galectins, formerly known as S-type lectins, are a family of β-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-9 (LGALS9) is a tandem repeat-type member of the galectin family. It has three isoforms (named galectin-9L, galectin-9M, and galectin-9S): long type of 355 amino acids, medium type of 323 amino acids, and short type of 311 amino acids. Galectin-9 is ubiquitously expressed in a variety of tissues, including lymph nodes and spleen, and overexpressed in Hodgkin disease tissue. It is involved in chemoattraction, apoptosis, and the regulation of cell differentiation and has anti-allergic effects. It has been reported that galectin-9 is a ligand for Tim-3, through which galectin-9 can induce T-helper type 1 lymphocyte (Th1) death.

Protocols

Product Specific Protocols
WB protocol for LGALS9/Galectin-9 antibody 30409-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols