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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LEMD2 Polyclonal antibody

LEMD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29406-1-AP

Synonyms

dJ482C21.1, hLEM2, LEM domain containing 2, LEMD2

Validation Data Gallery

Applications:
  • WB analysis using 29406-1-AP

    WB analysis using 29406-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29406-1-AP (LEMD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 29406-1-AP

    IHC staining of human colon using 29406-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29406-1-AP (LEMD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 29406-1-AP

    IHC staining of human colon using 29406-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29406-1-AP (LEMD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 29406-1-AP

    IHC staining of mouse kidney using 29406-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29406-1-AP (LEMD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 29406-1-AP

    IHC staining of mouse kidney using 29406-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29406-1-AP (LEMD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, MCF-7 cells, U2OS cells, human testis tissue
Positive IHC detected inhuman colon tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

29406-1-AP targets LEMD2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LEMD2 fusion protein Ag28078 相同性解析による交差性が予測される生物種
Full Name LEM domain containing 2
Calculated molecular weight 503 aa, 57 kDa
Observed molecular weight57 kDa
GenBank accession numberBC051803
Gene symbol LEMD2
Gene ID (NCBI) 221496
RRIDAB_2918298
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LEMD2 antibody 29406-1-APDownload protocol
IHC protocol for LEMD2 antibody 29406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols