Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, SKOV-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
10113-2-AP targets LDOC1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | LDOC1 fusion protein Ag0158 相同性解析による交差性が予測される生物種 |
| Full Name | leucine zipper, down-regulated in cancer 1 |
| Calculated molecular weight | 17 kDa |
| Observed molecular weight | 17 kDa, 25 kDa |
| GenBank accession number | BC003104 |
| Gene Symbol | LDOC1 |
| Gene ID (NCBI) | 23641 |
| RRID | AB_2135421 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95751 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LDOC1 contains a leucine zipper-like motif and a proline-rich region that shares marked similarity with an SH3-binding domain. LDOC1 localizes to the nucleus and is down-regulated in some cancer cell lines. LDOC1 regulates the transcriptional response mediated by the nuclear factor B (NF-kappa B). The gene encoding LDOC1 protein has been proposed as a tumor suppressor gene which may have an important role in the development and/or progression of some cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for LDOC1 antibody 10113-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Genet Leucine zipper downregulated in cancer 1 may serve as a favorable prognostic biomarker by influencing proliferation, colony formation, cell cycle, apoptosis, and migration ability in hepatocellular carcinoma |