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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LDHD Polyclonal antibody (14398-1-AP)

    WB analysis using 14398-1-AP (same clone as 14398-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14398-1-AP (LDHD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using LDHD Polyclonal antibody (14398-1-AP)

    WB analysis of HepG2 using 14398-1-AP (same clone as 14398-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14398-1-AP (LDHD antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LDHD Polyclonal antibody (14398-1-AP)

    IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LDHD Polyclonal antibody (14398-1-AP)

    IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LDHD Polyclonal antibody (14398-1-AP)

    IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LDHD Polyclonal antibody (14398-1-AP)

    IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human osteosarcoma tissue using LDHD Polyclonal antibody (14398-1-AP)

    IHC staining of human osteosarcoma using 14398-1-AP (same clone as 14398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14398-1-AP (LDHD antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human osteosarcoma tissue using LDHD Polyclonal antibody (14398-1-AP)

    IHC staining of human osteosarcoma using 14398-1-AP (same clone as 14398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14398-1-AP (LDHD antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LDHD Polyclonal antibody (14398-1-AP)

    IF Staining of HepG2 using 14398-1-AP (same clone as 14398-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using LDHD antibody 14398-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

LDHD Polyclonal antibody, PBS Only

LDHD Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14398-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14398-1-PBS targets LDHD in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5897

Product name: Recombinant human LDHD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 159-507 aa of BC047902

Sequence: GADASLCGMAATGASGTNAVRYGTMRDNVLNLEVVLPDGRLLHTAGRGRHFRFGFWPEIPHHTAWYSPCVSLGRRKSAAGYNLTGLFVGSEGTLGLITATTLRLHPAPEATVAATCAFPSVQAAVDSTVHILQAAVPVARIEFLDEVMMDACNRYSKLNCLVAPTLFLEFHGSQQALEEQLQRTEEIVQQNGASDFSWAKEAEERSRLWTARHNAWYAALATRPGCKGYSTDVCVPISRLPEIVVQTKEDLNASGLTGSIVGHVGDGNFHCILLVNPDDAEELGRVKAFAEQLGRRALALHGTCTGEHGIGMGKRQLLQEEVGAVGVETMRQLKAVLDPQGLMNPGKVL

相同性解析による交差性が予測される生物種
Full Name lactate dehydrogenase D
Calculated molecular weight 52 kDa
Observed molecular weight 45-54 kDa
GenBank accession numberBC047902
Gene Symbol LDHD
Gene ID (NCBI) 197257
RRIDAB_2249831
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86WU2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Two naturally occurring forms of lactate dehydrogenase with similar but unique substrate specificities have been isolated in lower organisms including invertebrates, fungi, and prokaryotes. These dehydrogenase enzymes are L-lactate dehydrogenase and D-lactate dehydrogenase (LDHD) that are specific to the L and D isomers of lactate, respectively (PMID: 12127981). In lactic acid bacteria, LDHD plays a key role in anaerobic energy metabolism (PMID: 497162). Despite the identification of D-lactate and other D-2-hydroxyacids in prokaryotes, and the obvious connections and similarities to vertebrate metabolic pathways, very few mammalian D-2-hydroxyacid dehydrogenases have been found. LDHD has 2 isoforms with the molecular weight of 52 and 54kDa, and can be detected as 45-54 kDa.

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