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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LDHC Polyclonal antibody

LDHC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14546-1-AP

Synonyms

Cancer/testis antigen 32, CT32, lactate dehydrogenase C, LDH C, LDH testis subunit, LDH X, LDH3, LDHC, LDHX

Validation Data Gallery

Applications:
  • WB analysis using 14546-1-AP

    WB analysis using 14546-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14546-1-AP (LDHC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 14546-1-AP

    IHC staining of mouse testis using 14546-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14546-1-AP (LDHC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 14546-1-AP

    IHC staining of mouse testis using 14546-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14546-1-AP (LDHC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, PC-3 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14546-1-AP targets LDHC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LDHC fusion protein Ag6050 相同性解析による交差性が予測される生物種
Full Name lactate dehydrogenase C
Calculated molecular weight 36 kDa
Observed molecular weight31-35 kDa
GenBank accession numberBC064388
Gene symbol LDHC
Gene ID (NCBI) 3948
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LDHC, also named as CT32 and LDH-X, belongs to the LDH/MDH superfamily and LDH family. LDHC is a possible role in sperm motility. LDHC catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. LDHC is a testis-specific isoform. Abnormal LDHC expression is associated with several forms of cancer. The antibody has no cross reaction with LDHA and LDHB.

Protocols

Product Specific Protocols
WB protocol for LDHC antibody 14546-1-APDownload protocol
IHC protocol for LDHC antibody 14546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols