Validation Data Gallery
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Tested Applications
| Positive WB detected in | DU 145 cells, HeLa cells, HEK-293T cells, Jurkat cells, PC-3 cells, mouse heart tissue, rat heart tissue, mouse brain tissue, mouse brain cortex tissue, mouse cerebellum tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
19988-1-AP targets LDHB-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | lactate dehydrogenase B |
| Calculated molecular weight | 37 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | NM_002300 |
| Gene Symbol | LDHB |
| Gene ID (NCBI) | 3945 |
| RRID | AB_10638780 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07195 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
LDHB, also named as NY-REN-46 and LDH-H, belongs to the LDH/MDH superfamily and LDH family. LDHB catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. It is glycolytic enzymes and it is positively regulated by mTOR.Defects in LDHB are a cause of hereditary LDHB deficiency. The antibody is specific to LDHB and has no cross reaction with LDHA and LDHC.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LDHB-Specific antibody 19988-1-AP | Download protocol |
| IHC protocol for LDHB-Specific antibody 19988-1-AP | Download protocol |
| WB protocol for LDHB-Specific antibody 19988-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Drug Resist Updat Lactate-driven H3K18 lactylation promotes cisplatin resistance in bladder cancer via HNRNPF-Parkin mediated mitophagy. | ||
Mol Cell Excess intracellular lactate represses mRNA translation and tumor progression through lactate-tRNA charging. | ||
EMBO Rep CDK1-mediated phosphorylation of LDHA fuels mitosis through LDHB-dependent lactate oxidation.
| ||
iScience Altered neuronal lactate dehydrogenase A expression affects cognition in a sex- and age-dependent manner | ||
iScience Lactate-driven type I collagen deposition facilitates cancer stem cell-like phenotype of head and neck squamous cell carcinoma
| ||
Biomedicines HMTM-Mediated Enhancement of Brain Bioenergetics in a Mouse Tauopathy Model Is Blocked by Chronic Administration of Rivastigmine. |