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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using LDHAL6A Polyclonal antibody (16221-1-AP)

    WB analysis of mouse liver using 16221-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16221-1-AP (LDHAL6A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using LDHAL6A Polyclonal antibody (16221-1-AP)

    WB analysis of Jurkat using 16221-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16221-1-AP (LDHAL6A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using LDHAL6A Polyclonal antibody (16221-1-AP)

    WB analysis of mouse testis using 16221-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16221-1-AP (LDHAL6A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using LDHAL6A Polyclonal antibody (16221-1-AP)

    WB analysis of mouse brain using 16221-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16221-1-AP (LDHAL6A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using LDHAL6A Polyclonal antibody (16221-1-AP)

    WB analysis of human brain using 16221-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16221-1-AP (LDHAL6A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LDHAL6A Polyclonal antibody (16221-1-AP)

    IF Staining of HeLa using 16221-1-AP

    Immunofluorescent analysis of Hela cells, using LDHAL6A antibody 16221-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

LDHAL6A Polyclonal antibody

LDHAL6A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16221-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

L-lactate dehydrogenase A-like 6A, LDHL2, LDHA-like protein 6A, EC:1.1.1.27

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, human brain tissue, Jurkat cells, mouse brain tissue, mouse testis tissue
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16221-1-AP targets LDHAL6A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9273

Product name: Recombinant human LDHAL6A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-234 aa of BC014340

Sequence: MATIKSELIKNFAEEEAIHHNKISIVGTGSVGVACAISILLKGLSDELVLVDVDEGKLKGETMDLQHGSPFMKMPNIVSSKDYLVTANSNLVIITAGARQKKGETRLDLVQRNVSIFKLMIPNITQYSPHCKLLIVTNPVDILTYVAWKLSGFPKNRVIGSGCNLDSARFRYFIGQRLGIHSESCHGLILGEHGDSSVPVWSGVNIAGVPLKDLNPDIGTDKDPEQWENVHKKK

相同性解析による交差性が予測される生物種
Full Name lactate dehydrogenase A-like 6A
Calculated molecular weight 26 kDa, 37 kDa
Observed molecular weight 30-36 kDa
GenBank accession numberBC014340
Gene Symbol LDHAL6A
Gene ID (NCBI) 160287
RRIDAB_2265629
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZMR3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for LDHAL6A antibody 16221-1-APDownload protocol
WB protocol for LDHAL6A antibody 16221-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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