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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using LCTL Polyclonal antibody (24589-1-AP)

    WB analysis of MCF-7 using 24589-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24589-1-AP (LCTL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LCTL Polyclonal antibody (24589-1-AP)

    IF Staining of HeLa using 24589-1-AP

    Immunofluorescent analysis of HeLa cells using 24589-1-AP (LCTL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

LCTL Polyclonal antibody

LCTL Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24589-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

KLG, KLPH, lactase like, Lactase like protein, LCTL

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24589-1-AP targets LCTL in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20220

Product name: Recombinant human LCTL protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 250-541 aa of BC152990

Sequence: SKQQGLVGISLNCDWGEPVDISNPKDLEAAERYLQFCLGWFANPIYAGDYPQVMKDYIGRKSAEQGLEMSRLPVFSLQEKSYIKGTSDFLGLGHFTTRYITERNYPSRQGPSYQNDRDLIELVDPNWPDLGSKWLYSVPWGFRRLLNFAQTQYGDPPIYVMENGASQKFHCTQLCDEWRIQYLKGYINEMLKAIKDGANIKGYTSWSLLDKFEWEKGYSDRYGFYYVEFNDRNKPRYPKASVQYYKKIIIANGFPNPREVESWYLKALETCSINNQMLAAEPLLSHMQMVTE

相同性解析による交差性が予測される生物種
Full Name lactase-like
Calculated molecular weight 567 aa, 65 kDa
Observed molecular weight 39 kDa, 65 kDa, 70 kDa
GenBank accession numberBC152990
Gene Symbol LCTL
Gene ID (NCBI) 197021
RRIDAB_2879625
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UWM7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lactase-like is a glycosidase enzyme, which could hydrolyze glycosidic bonds and are classified into families based on primary amino acid sequence. The mouse ortholog of this protein has been characterized and has a domain structure of an N-terminal signal peptide, glycosidase domain, transmembrane domain, and a short cytoplasmic tail.

Protocols

Product Specific Protocols
WB protocol for LCTL antibody 24589-1-APDownload protocol
IF protocol for LCTL antibody 24589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols