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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse thymus tissue using LCK Polyclonal antibody (24497-1-AP)

    WB analysis of mouse thymus using 24497-1-AP (same clone as 24497-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 24497-1-AP (LCK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24497-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using LCK Polyclonal antibody (24497-1-AP)

    WB analysis of Jurkat using 24497-1-AP (same clone as 24497-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24497-1-AP (LCK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24497-1-PBS in a different storage buffer formulation.

LCK Polyclonal antibody, PBS Only

LCK Polyclonal Antibody for WB, Indirect ELISA
Cat No. 24497-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24497-1-PBS targets LCK in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21475

Product name: Recombinant human LCK protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-539 aa of BC013200

Sequence: MGCGCSSHPEDDWMENIDVCENCHYPIVPLDGKGTLLIRNGSEVRDPLVTYEGSNPPASPLQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHELVRHYTNASDGLCTRLSRPCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHQRLVRLYAVVTQEPIYIITEYMENDTLLDSQLEEKGLGASPWGNLGQQLLLLPTGSLVDFLKTPSGIKLTINKLLDMAAQIAEGMAFIEERNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYQLMRLCWKERPEDRPTFDYLRSVLEDFFTATEGQYQPQP

相同性解析による交差性が予測される生物種
Full Name lymphocyte-specific protein tyrosine kinase
Calculated molecular weight 539 aa, 56 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC013200
Gene Symbol LCK
Gene ID (NCBI) 3932
RRIDAB_2879574
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP06239
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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