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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using LCE1B Polyclonal antibody (21771-1-AP)

    WB analysis of A375 using 21771-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 21771-1-AP (LCE1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using LCE1B Polyclonal antibody (21771-1-AP)

    WB analysis of mouse skin using 21771-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 21771-1-AP (LCE1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat skin tissue using LCE1B Polyclonal antibody (21771-1-AP)

    WB analysis of rat skin using 21771-1-AP

    rat skin tissue were subjected to SDS PAGE followed by western blot with 21771-1-AP (LCE1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using LCE1B Polyclonal antibody (21771-1-AP)

    IHC staining of human skin using 21771-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using LCE1B Polyclonal antibody (21771-1-AP)

    IHC staining of human skin using 21771-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using LCE1B Polyclonal antibody (21771-1-AP)

    IHC staining of human oesophagus using 21771-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using LCE1B Polyclonal antibody (21771-1-AP)

    IHC staining of human oesophagus using 21771-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using LCE1B Polyclonal antibody (21771-1-AP)

    IF Staining of A375 using 21771-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using LCE1B antibody (21771-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

LCE1B Polyclonal antibody

LCE1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21771-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

LEP2, SPRL2A, Small proline-rich-like epidermal differentiation complex protein 2A, Late envelope protein 2, late cornified envelope 1B

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells, mouse skin tissue, rat skin tissue
Positive IHC detected inhuman skin tissue, human oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21771-1-AP targets LCE1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16442

Product name: Recombinant human LCE1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-118 aa of BC104232

Sequence: MSCQQNQQQCQPPPKCIPKCPPKCLTPRCPPKCPPKCPPVSSCCSVSSGGCCGSSSGGSCGSSSGGCCSSGGGGCCLSHHRRRRSHCHRPQSSGCCSQPSGGSSCCGGGSGQHSGGCC

相同性解析による交差性が予測される生物種
Full Name late cornified envelope 1B
Calculated molecular weight 118 aa, 12 kDa
Observed molecular weight 10-12 kDa
GenBank accession numberBC104232
Gene Symbol LCE1B
Gene ID (NCBI) 353132
RRIDAB_10858482
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T7P3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human LCE1B (Late cornified envelope protein 1B), also named late envelope protein 2 (LEP2), is located at human Chromosome1q21 which contains multiple LCE clusters. LCE1B gene is predicated to encode a 118-aa protein, and is currently evidenced at transcript level. Its expression was skin-specific and was readily detected in adult trunk skin, adult arm skin, fetal skin, penal skin, vulva, esophagus and tongue (PMID: 15854049). LCE genes respond to environmental stimuli such as calcium levels and ultraviolet (UV) light, however, LCE groups have distinct functions (PMID: 15854049).

Protocols

Product Specific Protocols
IF protocol for LCE1B antibody 21771-1-APDownload protocol
IHC protocol for LCE1B antibody 21771-1-APDownload protocol
WB protocol for LCE1B antibody 21771-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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