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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LC8/DYNLL1 Polyclonal antibody (18130-1-AP)

    WB analysis using 18130-1-AP (same clone as 18130-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using LC8/DYNLL1 Polyclonal antibody (18130-1-AP)

    IP experiment of MCF-7 using 18130-1-AP (same clone as 18130-1-PBS)

    IP result of anti-LC8/DYNLL1 (IP:18130-1-AP, 4ug; Detection:18130-1-AP 1:1000) with MCF-7 cells lysate 640 ug. This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using LC8/DYNLL1 Polyclonal antibody (18130-1-AP)

    IHC staining of human liver using 18130-1-AP (same clone as 18130-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LC8/DYNLL1 Polyclonal antibody (18130-1-AP)

    IHC staining of mouse brain using 18130-1-AP (same clone as 18130-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LC8/DYNLL1 Polyclonal antibody (18130-1-AP)

    IHC staining of mouse brain using 18130-1-AP (same clone as 18130-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using LC8/DYNLL1 Polyclonal antibody (18130-1-AP)

    IHC staining of human liver using 18130-1-AP (same clone as 18130-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18130-1-AP (LC8/DYNLL1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using LC8/DYNLL1 Polyclonal antibody (18130-1-AP)

    IF Staining of MCF-7 using 18130-1-AP (same clone as 18130-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using LC8/DYNLL1 antibody (18130-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using LC8/DYNLL1 Polyclonal antibody (18130-1-AP)

    FC experiment of Jurkat using 18130-1-AP (same clone as 18130-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human LC8/DYNLL1 (18130-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 18130-1-PBS in a different storage buffer formulation.

LC8/DYNLL1 Polyclonal antibody, PBS Only

LC8/DYNLL1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 18130-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18130-1-PBS targets LC8/DYNLL1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12710

Product name: Recombinant human LC8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-89 aa of BC100289

Sequence: MCDRKAVIKNADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG

相同性解析による交差性が予測される生物種
Full Name dynein, light chain, LC8-type 1
Calculated molecular weight 89 aa, 10 kDa
Observed molecular weight8-10 kDa
GenBank accession numberBC100289
Gene Symbol DYNLL1
Gene ID (NCBI) 8655
RRIDAB_2923569
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP63167
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LC8 (also known as DYNLL1) is a homodimeric sequence-specific chaperone that promotes the ordered (typically homo-) oligomerization of more than a hundred protein targets. LC8/DYNLL1 has previously been proposed to function as an inhibitor of the NF-κB pathway, based on findings that it can bind directly to the IκBα regulatory region(PMID: 18579519). LC8/DYNLL1 regulates apoptotic activities of BCL2L11 by sequestering it to microtubules.

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