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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LBR Recombinant monoclonal antibody (84522-7-RR)

    IF Staining of HeLa using 84522-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LBR antibody (84522-7-RR, Clone: 241864G10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LBR Recombinant monoclonal antibody (84522-7-RR)

    IF Staining of HeLa using 84522-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LBR antibody (84522-7-RR, Clone: 241864G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LBR Recombinant antibody (84522-7-RR)

    IF Staining of HepG2 using 84522-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LBR antibody (84522-7-RR, Clone: 241864G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using LBR Recombinant monoclonal antibody (84522-7-RR)

    FC experiment of HeLa using 84522-7-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug LBR Recombinant antibody (84522-7-RR, Clone:241864G10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

LBR Recombinant monoclonal antibody

LBR Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 84522-7-RR
Clone No.241864G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

14, 241864G10, 3-beta-hydroxysterol Delta, C-14 sterol reductase, C14SR

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84522-7-RR targets LBR in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3088

Product name: Recombinant human LBR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-210 aa of BC020079

Sequence: MPSRKFADGEVVRGRWPGSSLYYEVEILSHDSTSQLYTVKYKDGTELELKENDIKPLTSFRQRKGGSTSSSPSRRRGSRSRSRSRSPGRPPKSARRSASASHQADIKEARREVEVKLTPLILKPFGNSISRYNGEPEHIERNDAPHKNTQEKFSLSQESSYIATQYSLRPRREEVKLKEIDSKEEKYVAKELAVRTFEVTPIRAKDLEFG

相同性解析による交差性が予測される生物種
Full Name lamin B receptor
Calculated molecular weight 615 aa, 71 kDa
GenBank accession numberBC020079
Gene Symbol LBR
Gene ID (NCBI) 3930
RRIDAB_3672030
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ14739
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lamin-B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that contains a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail (PMID: 28858257). LBR anchors the lamina and the heterochromatin to the inner nuclear membrane (PMID: 10828963; 28858257). It is also essential for cholesterol synthesis (PMID: 27336722). Mutations of the LBR gene have been associated with autosomal recessive HEM/Greenberg skeletal dysplasia and Pelger-Huët anomaly and Greenberg skeletal dysplasia (PMID: 12618959; 12490533).

Protocols

Product Specific Protocols
FC protocol for LBR antibody 84522-7-RRDownload protocol
IF protocol for LBR antibody 84522-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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