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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using LBP Polyclonal antibody (23559-1-AP)

    WB analysis of mouse liver using 23559-1-AP (same clone as 23559-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 23559-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23559-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using LBP Polyclonal antibody (23559-1-AP)

    WB analysis of mouse liver using 23559-1-AP (same clone as 23559-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 23559-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23559-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using LBP Polyclonal antibody (23559-1-AP)

    IP experiment of mouse liver using 23559-1-AP (same clone as 23559-1-PBS)

    IP result of anti-LBP (IP:23559-1-AP, 4ug; Detection:23559-1-AP 1:500) with mouse liver tissue lysate 3040 ug. This data was developed using the same antibody clone with 23559-1-PBS in a different storage buffer formulation.

LBP Polyclonal antibody, PBS Only

LBP Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 23559-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Synonyms

Lipopolysaccharide-binding protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23559-1-PBS targets LBP in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13485

Product name: Recombinant human LBP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 128-477 aa of BC022256

Sequence: SFDVSVKGISISVNLLLGSESSGRPTVTASSCSSDIADVEVDMSGDLGWLLNLFHNQIESKFQKVLESRICEMIQKSVSSDLQPYLQTLPVTTEIDSFADIDYSLVEAPRATAQMLEVMFKGEIFHRNHRSPVTLLAAVMSLPEEHNKMVYFAISDYVFNTASLVYHEEGYLNFSITDDMIPPDSNIRLTTKSFRPFVPRLARLYPNMNLELQGSVPSAPLLNFSPGNLSVDPYMEIDAFVLLPSSSKEPVFRLSVATNVSATLTFNTSKITGFLKPGKVKVELKESKVGLFNAELLEALLNYYILNTFYPKFNDKLAEGFPLPLLKRVQLYDLGLQIHKDFPSRRDHPP

相同性解析による交差性が予測される生物種
Full Name lipopolysaccharide binding protein
Calculated molecular weight 481 aa, 53 kDa
Observed molecular weight 53-60 kDa
GenBank accession numberBC022256
Gene Symbol LBP
Gene ID (NCBI) 3929
RRIDAB_11232597
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP18428
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LBP (lipopolysaccharide-binding protein) is an acute-phase protein, predominantly synthesized by hepatocytes. It is present in normal serum at concentrations of 5 to 10 μg/ml, rising up to 200 μg/ml 24 h after induction of an acute-phase response. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. (PMID: 9665271; 11598069)

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