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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LAYN Recombinant antibody (85900-1-RR)

    WB analysis using 85900-1-RR (same clone as 85900-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85900-1-RR (LAYN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85900-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using LAYN Recombinant antibody (85900-1-RR)

    IHC staining of mouse brain using 85900-1-RR (same clone as 85900-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85900-1-RR (LAYN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85900-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using LAYN Recombinant antibody (85900-1-RR)

    IF Staining of A549 using 85900-1-RR (same clone as 85900-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using LAYN antibody (85900-1-RR, Clone: 250175B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85900-1-PBS in a different storage buffer formulation.

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LAYN Recombinant antibody, PBS Only

LAYN Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85900-1-PBS
Clone No.250175B7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Layilin, UNQ208/PRO234

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85900-1-PBS targets LAYN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LAYN fusion protein Ag14508 相同性解析による交差性が予測される生物種
Full Name layilin
Calculated molecular weight 382 aa, 43 kDa
Observed molecular weight43 kDa
GenBank accession numberBC025407
Gene Symbol LAYN
Gene ID (NCBI) 143903
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6UX15
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LAYN acts as a receptor for hyaluronic acid (HA) and is involved in various cellular processes, including cell adhesion, movement, and signal transduction. In the context of cancer, LAYN has been identified as a prognostic biomarker. High expression of LAYN is associated with poor prognosis and increased immune cell infiltration in various cancers, such as colorectal cancer and gastric cancer. It may also play a role in T-cell exhaustion and the regulation of tumor-associated macrophages (PMID: 30761122).