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Host
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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LAX1 Polyclonal antibody

LAX1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21557-1-AP

Synonyms

LAX, LAX1

Validation Data Gallery

Applications:
  • WB analysis using 21557-1-AP

    WB analysis using 21557-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21557-1-AP (LAX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 21557-1-AP

    WB analysis of HeLa using 21557-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21557-1-AP (LAX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 21557-1-AP

    WB analysis of HeLa using 21557-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21557-1-AP (LAX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 21557-1-AP

    IHC staining of human spleen using 21557-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 21557-1-AP (LAX1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human spleen using 21557-1-AP

    IHC staining of human spleen using 21557-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 21557-1-AP (LAX1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 21557-1-AP

    IF Staining of HeLa using 21557-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 21557-1-AP (LAX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 21557-1-AP is LAX1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAX1 fusion protein Ag15599 相同性解析による交差性が予測される生物種
Full Name lymphocyte transmembrane adaptor 1
Calculated molecular weight 398 aa, 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC069650
Gene symbol LAX1
Gene ID (NCBI) 54900
RRIDAB_10732821
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LAX1 antibody 21557-1-APDownload protocol
IHC protocol for LAX1 antibody 21557-1-APDownload protocol
IF protocol for LAX1 antibody 21557-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Immunol

Mettl14-Mediated m6A Modification Is Essential for Germinal Center B Cell Response.

Authors - Hengjun Huang
View Article
humanWB

Cell Rep Med

A target discovery pipeline identified ILT3 as a target for immunotherapy of multiple myeloma

Authors - Francesco Di Meo
View Article