LAT Recombinant antibody

LAT Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2P13

Cat no : 82901-1-RR

Synonyms

LAT, LAT1, p36 38, pp36



Tested Applications

Positive WB detected inJurkat cells, EL-4 cells, human platelets
Positive IF detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)IF : 1:750-1:3000
Sample-dependent, check data in validation data gallery

Product Information

82901-1-RR targets LAT in WB, IF, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name linker for activation of T cells
Calculated molecular weight 262 aa, 28 kDa
Observed molecular weight 36-38 kDa
GenBank accession numberBC011563
Gene symbol LAT
Gene ID (NCBI) 27040
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LAT is a membrane-localized adaptor protein which is primarily concentrated in microdomains by palmitoylation. LAT plays a central role in T-cell activation by nucleating signaling complexes that are critical for the propagation of T-cell signals from the plasma membrane to the cellular interior. In addition to being a positive regulator of T cell signaling, LAT also recruits several negative regulatory proteins, including kinases, phosphatases, and ubiquitin ligases, which ultimately leads to signal termination. LAT is subject to ubiquitylation, and ubiquitin-resistant mutants of LAT display enhanced signaling.

Protocols

Product Specific Protocols
WB protocol for LAT antibody 82901-1-RRDownload protocol
IF protocol for LAT antibody 82901-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols