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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LASP1 Monoclonal antibody (68080-1-Ig)

    WB analysis using 68080-1-Ig (same clone as 68080-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68080-1-Ig (LASP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LASP1 Monoclonal antibody (68080-1-Ig)

    WB analysis using 68080-1-Ig (same clone as 68080-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68080-1-Ig (LASP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human peripheral blood platelets using LASP1 Monoclonal antibody (68080-1-Ig)

    WB analysis of human peripheral blood platelets using 68080-1-Ig (same clone as 68080-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 68080-1-Ig (LASP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using LASP1 Monoclonal antibody (68080-1-Ig)

    IHC staining of human breast cancer using 68080-1-Ig (same clone as 68080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LASP1 Monoclonal antibody (68080-1-Ig)

    IHC staining of human liver cancer using 68080-1-Ig (same clone as 68080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LASP1 Monoclonal antibody (68080-1-Ig)

    IHC staining of human liver cancer using 68080-1-Ig (same clone as 68080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using LASP1 Monoclonal antibody (68080-1-Ig)

    IHC staining of human breast cancer using 68080-1-Ig (same clone as 68080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using LASP1 Monoclonal antibody (68080-1-Ig)

    IF Staining of A549 using 68080-1-Ig (same clone as 68080-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using LASP1 antibody (68080-1-Ig, Clone: 1G4B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

LASP1 Monoclonal antibody, PBS Only

LASP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68080-1-PBS
Clone No.1G4B6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

1G4B6, LASP-1, LIM and SH3 protein 1, MLN50

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68080-1-PBS targets LASP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag18101

Product name: Recombinant human LASP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 125-210 aa of BC012460

Sequence: EFEKSRMGPSGGEGMEPERRDSQDGSSYRRPLEQQQPHHIPTSAPVYQQPQQQPVAQSYGGYKEPAAPVSIQRSAPGGGGKRYRAA

相同性解析による交差性が予測される生物種
Full Name LIM and SH3 protein 1
Calculated molecular weight 30 kDa
Observed molecular weight35-38 kDa
GenBank accession numberBC012460
Gene Symbol LASP1
Gene ID (NCBI) 3927
ENSEMBL Gene IDENSG00000002834
RRIDAB_2918817
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ14847
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LASP1(LIM and SH3 protein 1), also known as MLN50, is a 261 amino acid protein that localizes to both the cytoplasm and the cytoskeleton(PMID: 7589475). LASP1 consists of an N-terminal LIM-domain with two zinc finger motifs, followed by two central actin-binding nebulin repeats, flanked by a linker region and a C-terminal SH3 domain (PMID: 17177073, 9848085). LASP-1 interacts with F-Actin and plays an important role in the regulation of Actin-associated cytoskeletal organization. Agonist-dependent changes in LASP1 phosphorylation may regulate Actin-related ion transport activities in epithelial cells (PMID: 15465019,12571245). Overexpression of LASP-1 is associated with breast cancer, and plays a role in tumor transformation and metastasis (PMID: 17956604).

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