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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LARS Monoclonal antibody

LARS Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E8D4

Cat no : 67940-1-Ig

Synonyms

FLJ10595, FLJ21788, hr025Cl, HSPC192, KIAA1352, LARS, LARS1, Leucine tRNA ligase, leucyl tRNA synthetase, LEURS, LEUS, LRS, PIG44, RNTLS

Validation Data Gallery

Applications:
  • WB analysis using 67940-1-Ig

    WB analysis using 67940-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67940-1-Ig (LARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • WB analysis using 67940-1-Ig

    WB analysis using 67940-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67940-1-Ig (LARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67940-1-Ig

    IF Staining of HeLa using 67940-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LARS antibody (67940-1-Ig, Clone: 1E8D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, PC-12 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, 4T1 cells
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LARS1 Ready-To-Use IHC Kit. LARS1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67940-1-Ig targets LARS in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LARS fusion protein Ag15944 相同性解析による交差性が予測される生物種
Full Name leucyl-tRNA synthetase
Calculated molecular weight 1176 aa, 134 kDa
Observed molecular weight 135-140 kDa
GenBank accession numberBC151214
Gene symbol LARS
Gene ID (NCBI) 51520
RRIDAB_2918692
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LARS antibody 67940-1-IgDownload protocol
IF protocol for LARS antibody 67940-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols