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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LARS Polyclonal antibody (21146-1-AP)

    WB analysis using 21146-1-AP (same clone as 21146-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21146-1-AP (LARS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21146-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using LARS Polyclonal antibody (21146-1-AP)

    WB analysis of HeLa using 21146-1-AP (same clone as 21146-1-PBS)

    WB result of LARS antibody (21146-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LARS transfected HeLa cells. This data was developed using the same antibody clone with 21146-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using LARS Polyclonal antibody (21146-1-AP)

    IP experiment of Jurkat using 21146-1-AP (same clone as 21146-1-PBS)

    IP result of anti-LARS (IP:21146-1-AP, 4ug; Detection:21146-1-AP 1:500) with Jurkat cells lysate 2400ug. This data was developed using the same antibody clone with 21146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using LARS Polyclonal antibody (21146-1-AP)

    IHC staining of human colon using 21146-1-AP (same clone as 21146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21146-1-AP (LARS Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 21146-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using LARS Polyclonal antibody (21146-1-AP)

    IHC staining of human colon using 21146-1-AP (same clone as 21146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21146-1-AP (LARS Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 21146-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LARS Polyclonal antibody (21146-1-AP)

    IF Staining of HeLa using 21146-1-AP (same clone as 21146-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LARS antibody (21146-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21146-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

LARS Polyclonal antibody, PBS Only

LARS Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 21146-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21146-1-PBS targets LARS in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15684

Product name: Recombinant human LARS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 827-1176 aa of BC151214

Sequence: EFQAAKDKYRELAVEGMHRELVFRFIEVQTLLLAPFCPHLCEHIWTLLGKPDSIMNASWPVAGPVNEVLIHSSQYLMEVTHDLRLRLKNYMMPAKGKKTDKQPLQKPSHCTIYVAKNYPPWQHTTLSVLRKHFEANNGKLPDNKVIASELGSMPELKKYMKKVMPFVAMIKENLEKMGPRILDLQLEFDEKAVLMENIVYLTNSLELEHIEVKFASEAEDKIREDCCPGKPLNVFRIEPGVSVSLVNPQPSNGHFSTKIEIRQGDNCDSIIRRLMKMNRGIKDLSKVKLMRFDDPLLGPRRVPVLGKEYTEKTPISEHAVFNVDLMSKKIHLTENGIRVDIGDTIIYLVH

相同性解析による交差性が予測される生物種
Full Name leucyl-tRNA synthetase
Calculated molecular weight 1176 aa, 134 kDa
Observed molecular weight 135-140 kDa
GenBank accession numberBC151214
Gene Symbol LARS
Gene ID (NCBI) 51520
RRIDAB_10733878
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2J5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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