Validation Data Gallery

Filter: AllWB

WB analysis of mouse kidney using 24307-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24307-1-AP (LARGE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 24307-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 24307-1-AP (LARGE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 24307-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24307-1-AP (LARGE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 24307-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24307-1-AP (LARGE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

LARGE Polyclonal antibody

LARGE Polyclonal Antibody for WB, ELISA

Cat No. 24307-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

KIAA0609, LARGE, LARGE1, like glycosyltransferase, MDC1D

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in

mouse kidney tissue, mouse brain tissue, mouse skeletal muscle tissue, NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24307-1-AP targets LARGE in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag19458 Product name: Recombinant human LARGE protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 33-190 aa of BC126404 Sequence: SFEDGKPVSLSPLESQAHSPRYTASSQRERESLEVRMREVEEENRALRRQLSLAQGRAPSHRRGNHSKTYSMEEGTGDSENLRAGIVAGNSSECGQQPVVEKCETIHVAIVCAGYNASRDVVTLVKSVLFHRRNPLHFHLIADSIAEQILATLFQTWM 相同性解析による交差性が予測される生物種
Full Name	like-glycosyltransferase
Calculated molecular weight	756 aa, 88 kDa
Observed molecular weight	65-70 kDa
GenBank accession number	BC126404
Gene Symbol	LARGE
Gene ID (NCBI)	9215
RRID	AB_2879486
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95461
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for LARGE antibody 24307-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

製品

アプリケーション

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サポート

カスタム対応

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企業情報

抗体

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse kidney using 24307-1-AP

WB analysis of mouse brain using 24307-1-AP

WB analysis of mouse skeletal muscle using 24307-1-AP

WB analysis of NIH/3T3 using 24307-1-AP