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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using LARG Polyclonal antibody (22441-1-AP)

    WB analysis of Jurkat using 22441-1-AP (same clone as 22441-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22441-1-AP (LARG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using LARG Polyclonal antibody (22441-1-AP)

    WB analysis of HeLa using 22441-1-AP (same clone as 22441-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 22441-1-AP (LARG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using LARG Polyclonal antibody (22441-1-AP)

    IP experiment of HEK-293 using 22441-1-AP (same clone as 22441-1-PBS)

    IP result of anti-LARG (IP:22441-1-AP, 4ug; Detection:22441-1-AP 1:4000) with HEK-293 cells lysate 1320 ug. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using LARG Polyclonal antibody (22441-1-AP)

    IHC staining of human testis using 22441-1-AP (same clone as 22441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22441-1-AP (LARG Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using LARG Polyclonal antibody (22441-1-AP)

    IHC staining of human testis using 22441-1-AP (same clone as 22441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22441-1-AP (LARG Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LARG Polyclonal antibody (22441-1-AP)

    IF Staining of HepG2 using 22441-1-AP (same clone as 22441-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using ARHGEF12 antibody 22441-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LARG Polyclonal antibody (22441-1-AP)

    IF Staining of HeLa using 22441-1-AP (same clone as 22441-1-PBS)

    Immunofluorescent analysis of HeLa cells using 22441-1-AP (LARG antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 22441-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

LARG Polyclonal antibody, PBS Only

LARG Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 22441-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ARHGEF12, KIAA0382, Leukemia associated RhoGEF, Leukemia-associated RhoGEF

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22441-1-PBS targets LARG in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18211

Product name: Recombinant human LARG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 214-563 aa of BC063117

Sequence: QRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDASRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSM

相同性解析による交差性が予測される生物種
Full Name Rho guanine nucleotide exchange factor (GEF) 12
Calculated molecular weight 1544 aa, 173 kDa
Observed molecular weight 220 kDa
GenBank accession numberBC063117
Gene Symbol LARG
Gene ID (NCBI) 23365
RRIDAB_2879107
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZN5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LARG, also known as ARHGEF12, is one of three regulator of G protein signalling (RGS) domain-containing RhoGEFs. LARG activates the ROCK pathway downstream of Gα12/13 signaling, leading to cytoskeletal reorganisation. LARG performs this activity either as a homodimer or as a heterodimer with Arhgef11. LARG has been implicated in many diseases, including cancer, heart disease, and in HIV viral replication. LARG is phosphorylated and dephosphorylated during mitosis and that Cdk1 is the mitotic kinase responsible for LARG phosphorylation. We got 220 kDa in western blotting, maybe due to its phosphorylation.

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