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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lymph tissue using LAPTM4B Polyclonal antibody (18895-1-AP)

    WB analysis of mouse lymph using 18895-1-AP (same clone as 18895-1-PBS)

    mouse lymph tissue were subjected to SDS PAGE followed by western blot with 18895-1-AP (LAPTM4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18895-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat lymph tissue using LAPTM4B Polyclonal antibody (18895-1-AP)

    WB analysis of rat lymph using 18895-1-AP (same clone as 18895-1-PBS)

    rat lymph tissue were subjected to SDS PAGE followed by western blot with 18895-1-AP (LAPTM4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18895-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LAPTM4B Polyclonal antibody (18895-1-AP)

    IHC staining of human liver cancer using 18895-1-AP (same clone as 18895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18895-1-AP (LAPTM4B Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18895-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LAPTM4B Polyclonal antibody (18895-1-AP)

    IHC staining of human liver cancer using 18895-1-AP (same clone as 18895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18895-1-AP (LAPTM4B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18895-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LAPTM4B Polyclonal antibody (18895-1-AP)

    IHC staining of human liver cancer using 18895-1-AP (same clone as 18895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18895-1-AP (LAPTM4B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18895-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using LAPTM4B Polyclonal antibody (18895-1-AP)

    IHC staining of human heart using 18895-1-AP (same clone as 18895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18895-1-AP (LAPTM4B Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18895-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using LAPTM4B Polyclonal antibody (18895-1-AP)

    IHC staining of human heart using 18895-1-AP (same clone as 18895-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18895-1-AP (LAPTM4B Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18895-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

LAPTM4B Polyclonal antibody, PBS Only

LAPTM4B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 18895-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18895-1-PBS targets LAPTM4B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAPTM4B fusion protein Ag13521 相同性解析による交差性が予測される生物種
Full Name lysosomal protein transmembrane 4 beta
Calculated molecular weight 41 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC014129
Gene Symbol LAPTM4B
Gene ID (NCBI) 55353
RRIDAB_10597385
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86VI4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

LAPTM4B, a novel oncoprotein, is associated with the prognosis of cancer, and may play important roles in the initiation, promotion and metastasis of tumors. It was initially identified as a hepatocellular carcinoma-associated gene, and was recently shown to be up-regulated in various human cancers. Some studies indicated that LAPTM4B may be an useful marker of prognosis in ovarian carcinoma and endometrial cancer. LAPTM4B belongs to the LAPTM4/LAPTM5 transporter family. It has 3 isoforms with molecular masses 41 kDa, 35 kDa and 24 kDa. This antibody can recognize all the 3 isoforms of LAPTM4B.