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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LAPTM4A Polyclonal antibody (30627-1-AP)

    WB analysis using 30627-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30627-1-AP (LAPTM4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using LAPTM4A Polyclonal antibody (30627-1-AP)

    IF Staining of mouse kidney using 30627-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using LAPTM4A antibody (30627-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using LAPTM4A Polyclonal antibody (30627-1-AP)

    IF Staining of mouse kidney using 30627-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using LAPTM4A antibody (30627-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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LAPTM4A Polyclonal antibody

LAPTM4A Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 30627-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF-P, ELISA

Synonyms

HUMORF13, KIAA0108, LAPTM4, LAPTM4A, MBNT, Mtrp

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue
Positive IF-P detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30627-1-AP targets LAPTM4A in WB, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAPTM4A fusion protein Ag6501 相同性解析による交差性が予測される生物種
Full Name lysosomal protein transmembrane 4 alpha
Calculated molecular weight 27 kDa
Observed molecular weight70 kDa
GenBank accession numberBC000421
Gene Symbol LAPTM4A
Gene ID (NCBI) 9741
RRIDAB_3086376
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15012
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LAPTM4A is an intracellular membrane protein that regulates the function of organic cation transporter-2 (OCT2) through direct interaction. LAPTM4A can also form a complex with A4GALT in the Golgi and shows at about 70 kDa.

Protocols

Product Specific Protocols
WB protocol for LAPTM4A antibody 30627-1-APDownload protocol
IF protocol for LAPTM4A antibody 30627-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols