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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

LANCL1 Monoclonal antibody

LANCL1 Monoclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat , pig

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2H6E8

Cat no : 68160-1-Ig

Synonyms

GPR69A, LanC like protein 1, LANCL1, p40

Validation Data Gallery

Applications:
  • WB analysis using 68160-1-Ig

    WB analysis using 68160-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68160-1-Ig (LANCL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IP experiment of mouse brain using 68160-1-Ig

    IP experiment of mouse brain using 68160-1-Ig

    IP result of anti-LANCL1 (IP:68160-1-Ig, 5ug; Detection:68160-1-Ig 1:2000) with mouse brain tissue lysate 1600 ug.

  • IF Staining of U-251 using 68160-1-Ig

    IF Staining of U-251 using 68160-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using LANCL1 antibody (68160-1-Ig, Clone: 2H6E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IF detected inU-251 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

68160-1-Ig targets LANCL1 in WB, IP, IF, ELISA applications and shows reactivity with Human, mouse, rat , pig samples.

Tested Reactivity Human, mouse, rat , pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LANCL1 fusion protein Ag32665 相同性解析による交差性が予測される生物種
Full Name LanC lantibiotic synthetase component C-like 1 (bacterial)
Calculated molecular weight 399 aa, 45 kDa
Observed molecular weight40 kDa
GenBank accession numberBC028685
Gene symbol LANCL1
Gene ID (NCBI) 10314
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LanCL1 ( also known as P40 or GRP69A), is homologous to bacterial lanthionine synthetase C (LanC) family, which is involved in the biosynthesis of antimicrobial peptides (lantibiotics). LanCL1 was identified as a reduced glutathione (GSH)-binding protein and primarily expressed in neural tissues and testis.

Protocols

Product Specific Protocols
WB protocol for LANCL1 antibody 68160-1-IgDownload protocol
IF protocol for LANCL1 antibody 68160-1-IgDownload protocol
IP protocol for LANCL1 antibody 68160-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols