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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LAMB3 Recombinant antibody (85055-4-RR)

    WB analysis using 85055-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85055-4-RR (LAMB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85055-4-RR

    Affinity and Kinetic Characterization of 85055-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85055-4-RR against Human LAMB3 were performed. The affinity constant is 0.244 nM.

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LAMB3 Recombinant antibody

LAMB3 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85055-4-RR
Clone No.242442A8

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

Laminin B1k chain, Laminin 5 subunit beta, Kalinin subunit beta, Kalinin B1 chain, Epiligrin subunit bata

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, U2OS cells, rat lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85055-4-RR targets LAMB3 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LAMB3 fusion protein Ag25192 相同性解析による交差性が予測される生物種
Full Name laminin, beta 3
Calculated molecular weight 1172 aa, 130 kDa
Observed molecular weight130-140 kDa
GenBank accession numberBC075838
Gene Symbol LAMB3
Gene ID (NCBI) 3914
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13751
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LAMB3 antibody 85055-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols