Validation Data Gallery
Tested Applications
| Positive WB detected in | human heart tissue, human skeletal muscle tissue |
| Positive IHC detected in | human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 6 publications below |
Product Information
10465-1-AP targets LAMA4 (Isoform 3) in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0736 Product name: Recombinant human LAMA4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-120 aa of BC004241 Sequence: MALSSAWRSVLPLWLLWSAACSRAASGDDNAFPFDIEGSSAVGRQDPPETSEPRVALGRLPPAAEVQCPCHCHPAGAPAPPRAVPHSSFSLSPPLSSPQCLESFTWARSVRKLEIKSFPL 相同性解析による交差性が予測される生物種 |
| Full Name | laminin, alpha 4 |
| Calculated molecular weight | 13 kDa |
| Observed molecular weight | 11-13 kDa |
| GenBank accession number | BC004241 |
| Gene Symbol | Laminin alpha 4/LAMA4 |
| Gene ID (NCBI) | 3910 |
| RRID | AB_2234461 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16363 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Laminin is a basement membrane glycoprotein composed of three nonidentical chains, A, B1, and B2 (PMID: 7959779). LAMA4 (laminin, alpha 4), an A chain variant, is a subunit of laminin-8 (laminin-411), laminin-9 (laminin-421) and laminin-14 (laminin-423) (PMID: 10842354; 10964957). LAMA4 is widely distributed in developing and adult human tissues, and mainly localized to mesenchyme-derived tissues (PMID: 12133914). It may have a role in formation and function of endothelium, transmigration of inflammatory cells through endothelium, and invasion of certain tumors (PMID: 17533363). Three alternatively spliced mRNAs give rise to three isoforms. This antibody was generated against the full length (1-120aa) of isoform 3.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LAMA4 (Isoform 3) antibody 10465-1-AP | Download protocol |
| WB protocol for LAMA4 (Isoform 3) antibody 10465-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Rep Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer. | ||
Am J Physiol Heart Circ Physiol Pygo1 regulates pathological cardiac hypertrophy via a β-catenin-dependent mechanism. | ||
Neuromuscul Disord Basement membrane remodelling and segmental fibrosis in sporadic inclusion body myositis. | ||
J Neurosci Res Maturational changes in laminin, fibronectin, collagen IV, and perlecan in germinal matrix, cortex, and white matter and effect of betamethasone. | ||
Cancer Cell Int MiR-539 inhibits proliferation and migration of triple-negative breast cancer cells by down-regulating LAMA4 expression. |
