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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LAMA2 Polyclonal antibody (31181-1-AP)

    WB analysis using 31181-1-AP (same clone as 31181-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31181-1-AP (LAMA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31181-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using LAMA2 Polyclonal antibody (31181-1-AP)

    IHC staining of mouse skeletal muscle using 31181-1-AP (same clone as 31181-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 31181-1-AP (LAMA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31181-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using LAMA2 Polyclonal antibody (31181-1-AP)

    IHC staining of mouse heart using 31181-1-AP (same clone as 31181-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31181-1-AP (LAMA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31181-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using LAMA2 Polyclonal antibody (31181-1-AP)

    IF Staining of mouse skeletal muscle using 31181-1-AP (same clone as 31181-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using LAMA2 antibody (31181-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31181-1-PBS in a different storage buffer formulation.

LAMA2 Polyclonal antibody, PBS Only

LAMA2 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 31181-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

LAMM, Laminin-4 subunit alpha, Laminin-2 subunit alpha, Laminin-12 subunit alpha, Laminin subunit alpha-2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31181-1-PBS targets LAMA2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34494

Product name: Recombinant human LAMA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2100-2250 aa of NM_000426

Sequence: IDKLKPIKELEDNLKKNISEIKELINQARKQANSIKVSVSSGGDCIRTYKPEIKKGSYNNIVVNVKTAVADNLLFYLGSAKFIDFLAIEMRKGKVSFLWDVGSGVGRVEYPDLTIDDSYWYRIVASRTGRNGTISVRALDG

相同性解析による交差性が予測される生物種
Full Name laminin, alpha 2
Calculated molecular weight344 kDa
Observed molecular weight344 kDa
GenBank accession numberNM_000426
Gene Symbol LAMA2
Gene ID (NCBI) 3908
RRIDAB_3669885
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDP24043
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The laminin subunit alpha 2 (LAMA2) gene encodes an alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Basal laminae are structural components of the extracellular matrix that influence cell proliferation and differentiation,7 and laminin subunit alpha 2 (LAMA2) is the major component of the basal laminae-α subunit of laminin. (PMID: 30728939)

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