• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Immunoprecipitation (IP) experiment of mouse liver tissue using LAG-3/CD223 Polyclonal antibody (16616-1-AP)

    IP experiment of mouse liver using 16616-1-AP (same clone as 16616-1-PBS)

    IP result of anti-LAG-3 (IP:16616-1-AP, 4ug; Detection:16616-1-AP 1:500) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using LAG-3/CD223 Polyclonal antibody (16616-1-AP)

    IHC staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16616-1-AP (LAG-3/CD223 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using LAG-3/CD223 Polyclonal antibody (16616-1-AP)

    IHC staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16616-1-AP (LAG-3/CD223 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using LAG-3/CD223 Polyclonal antibody (16616-1-AP)

    IF Staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LAG-3 antibody (16616-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using LAG-3/CD223 Polyclonal antibody (16616-1-AP)

    IF Staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LAG-3 antibody (16616-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

LAG-3/CD223 Polyclonal antibody, PBS Only

LAG-3/CD223 Polyclonal Antibody for IHC, IF-P, IP, ELISA
Cat No. 16616-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, IP, ELISA

Synonyms

LAG-3, CD223, lymphocyte activation gene 3, LAG3, LAG 3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16616-1-PBS targets LAG-3/CD223 in IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAG-3/CD223 fusion protein Ag9909 相同性解析による交差性が予測される生物種
Full Name lymphocyte-activation gene 3
Calculated molecular weight 525 aa, 57 kDa
Observed molecular weight 54 kDa, 60-65 kDa
GenBank accession numberBC052589
Gene Symbol LAG-3
Gene ID (NCBI) 3902
RRIDAB_2133350
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18627
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)