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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LACTB Monoclonal antibody (66785-1-Ig)

    WB analysis using 66785-1-Ig (same clone as 66785-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66785-1-Ig (LACTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66785-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LACTB Monoclonal antibody (66785-1-Ig)

    IHC staining of human liver cancer using 66785-1-Ig (same clone as 66785-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66785-1-Ig (LACTB antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66785-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LACTB Monoclonal antibody (66785-1-Ig)

    IHC staining of human liver cancer using 66785-1-Ig (same clone as 66785-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66785-1-Ig (LACTB antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66785-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using LACTB Monoclonal antibody (66785-1-Ig)

    IF Staining of mouse liver using 66785-1-Ig (same clone as 66785-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using LACTB antibody (66785-1-Ig, Clone: 3C3C3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66785-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using LACTB Monoclonal antibody (66785-1-Ig)

    IF Staining of A549 using 66785-1-Ig (same clone as 66785-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using LACTB antibody (66785-1-Ig, Clone: 3C3C3 ) at dilution of 1:1600 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66785-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LACTB Monoclonal antibody (66785-1-Ig)

    IF Staining of HepG2 using 66785-1-Ig (same clone as 66785-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66785-1-Ig (LACTB antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66785-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

LACTB Monoclonal antibody, PBS Only

LACTB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66785-1-PBS
Clone No.3C3C3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

3C3C3, EC:3.4.-.-, G24, lactamase, beta, MRPL56

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66785-1-PBS targets LACTB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag12233

Product name: Recombinant human LACTB protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 73-373 aa of BC067288

Sequence: DPEASPLAEPPQEQSLAPWSPQTPAPPCSRCFARAIESSRDLLHRIKDEVGAPGIVVGVSVDGKEVWSEGLGYADVENRVPCKPETVMRIASISKSLTMVALAKLWEAGKLDLDIPVQHYVPEFPEKEYEGEKVSVTTRLLISHLSGIRHYEKDIKKVKEEKAYKALKMMKENVAFEQEKEGKSNEKNDFTKFKTEQENEAKCRNSKPGKKKNDFEQGELYLREKFENSIESLRLFKNDPLFFKPGSQFLYSTFGYTLLAAIVERASGCKYLDYMQKIFHDLDMLTTVQEENEPVIYNRAR

相同性解析による交差性が予測される生物種
Full Name lactamase, beta
Calculated molecular weight 547 aa, 61 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC067288
Gene Symbol LACTB
Gene ID (NCBI) 114294
RRIDAB_2882130
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP83111
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

LACTB is also named as MRPL56,whic is a component of the large subunit of the mitochondrial ribosome that is encoded by the nuclear genome.LACTB is a mammalian active-site serine protein that has evolved from a bacterial penicillin-binding protein.It promotes intramitochondrial membrane organization and micro-compartmentalization through polymerization(PMID:19858488). Northern blot analysis of mouse tissues detected expression at high levels in liver, heart, and skeletal muscle, with lower levels in kidney, testis, brain, lung, and spleen(PMID:11707067). The full-length of LACTB is about 61kDa, and a 115aa transit peptide can be removed in the mature form.

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