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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using L3MBTL3 Polyclonal antibody (28112-1-AP)

    WB analysis using 28112-1-AP (same clone as 28112-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28112-1-AP (L3MBTL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using L3MBTL3 Polyclonal antibody (28112-1-AP)

    IHC staining of mouse cerebellum using 28112-1-AP (same clone as 28112-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28112-1-AP (L3MBTL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using L3MBTL3 Polyclonal antibody (28112-1-AP)

    IHC staining of mouse cerebellum using 28112-1-AP (same clone as 28112-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28112-1-AP (L3MBTL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using L3MBTL3 Polyclonal antibody (28112-1-AP)

    IHC staining of mouse cerebellum using 28112-1-AP (same clone as 28112-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28112-1-AP (L3MBTL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using L3MBTL3 Polyclonal antibody (28112-1-AP)

    IHC staining of mouse cerebellum using 28112-1-AP (same clone as 28112-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28112-1-AP (L3MBTL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using L3MBTL3 Polyclonal antibody (28112-1-AP)

    IF Staining of U2OS using 28112-1-AP (same clone as 28112-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using L3MBTL3 antibody (28112-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

L3MBTL3 Polyclonal antibody, PBS Only

L3MBTL3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28112-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28112-1-PBS targets L3MBTL3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27136

Product name: Recombinant human L3MBTL3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 524-646 aa of BC060845

Sequence: LMEASEHGGCSTPGCKGIGHFKRARHLGPHSAANCPYSEINLNKDRIFPDRLSGEMPPASPSFPRNKRTDANESSSSPEIRDQHADDVKEDFEERTESEMRTSHEARGAREEPTVQQAQRRSA

相同性解析による交差性が予測される生物種
Full Name l(3)mbt-like 3 (Drosophila)
Calculated molecular weight 88 aa, 3 kDa
Observed molecular weight88 kDa
GenBank accession numberBC060845
Gene Symbol L3MBTL3
Gene ID (NCBI) 84456
RRIDAB_2918148
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JM7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

L3MBTL3, also named as MBT1 and KIAA1798, belongs to the Malignant brain tumor (MBT) family. L3MBTL3 can bind to methyl lysine in histone, further affecting the transcription and its abnormal regulation associated with many diseases (PMID: 34373992). Additionally, researches have shown that L3MBTL3 plays a role in meduloblastoma formation and normal hematopoiesis in humans (PMID: 26317848). L3MBTL3 has 2 isoforms with the molecular mass of 86 and 88 kDa.

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