Validation Data Gallery
Tested Applications
| Positive WB detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24435-1-AP targets L3MBTL2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19571 Product name: Recombinant human L3MBTL2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 284-427 aa of BC017191 Sequence: RTIHAKFTDWKGYLMKRLVGSRTLPVDFHIKMVESMKYPFRQGMRLEVVDKSQVSRTRMAVVDTVIGGRLRLLYEDGDSDDDFWCHMWSPLIHPVGWSRRVGHGIKMSERRSDMAHHPTFRKIYCDAVPYLFKKVRAVYTEGGW 相同性解析による交差性が予測される生物種 |
| Full Name | l(3)mbt-like 2 (Drosophila) |
| Calculated molecular weight | 705 aa, 79 kDa |
| Observed molecular weight | 95-100 kDa |
| GenBank accession number | BC017191 |
| Gene Symbol | L3MBTL2 |
| Gene ID (NCBI) | 83746 |
| RRID | AB_3085733 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q969R5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
L3MBTL2 (Lethal (3) malignant brain tumor like 2) is a member of the MBT-domain proteins, which are involved in transcriptional repression and implicated in chromatin compaction. L3MBTL2 was most highly expressed in pachytene spermatocytes within the testis (PMID: 30760872).In addition, mutations in L3MBTL2 are prevalent in various cancers including leukemia, a disease characterized by alterations in multiple DNA repair proteins (PMID: 29581593).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for L3MBTL2 antibody 24435-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |