Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, PC-3 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33146-1-AP targets L3HYPDH in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37121 Product name: Recombinant human C14orf149 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 195-354 aa of BC012131 Sequence: VTAEKLGLDICSAKTRDLVDAASAVTEAVKAQFKINHPDSEDLAFLYGTILTDGKDAYTKEPTTNICVFADEQVDRSPTGSGVTARIALQYHKGLLELNQMRAFKSSATGSVFTGKAVREAKCGDFKAVIVEVSGQAHYTGTASFIIEDDDPLRDGFLLK 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 14 open reading frame 149 |
| Calculated molecular weight | 38 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC012131 |
| Gene Symbol | C14orf149 |
| Gene ID (NCBI) | 112849 |
| RRID | AB_3742871 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96EM0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Trans-3-hydroxy-L-proline dehydratase (L3HYPDH) is an enzyme that catalyzes the conversion of trans-3-hydroxy-L-proline to delta(1)-pyrroline-2-carboxylate. This enzyme is involved in the degradation of dietary proteins containing trans-3-hydroxy-L-proline, such as collagen IV. Additionally, L3HYPDH has been identified as an interferon-stimulated gene (ISG) with antiviral activity, particularly against viruses like EV71 (PMID: 35891782). It also plays a role in the genetic regulation of working memory and has been implicated in the mitochondrial pathway associated with autism spectrum disorder (PMID: 32490597).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for L3HYPDH antibody 33146-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |