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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using L1CAM Polyclonal antibody (32885-1-AP)

    WB analysis using 32885-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32885-1-AP (L1CAM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using L1CAM Polyclonal antibody (32885-1-AP)

    IHC staining of mouse cerebellum using 32885-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32885-1-AP (L1CAM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using L1CAM Polyclonal antibody (32885-1-AP)

    IHC staining of mouse cerebellum using 32885-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32885-1-AP (L1CAM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using L1CAM Polyclonal antibody (32885-1-AP)

    IHC staining of mouse brain using 32885-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32885-1-AP (L1CAM antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using L1CAM Polyclonal antibody (32885-1-AP)

    IHC staining of mouse brain using 32885-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32885-1-AP (L1CAM antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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L1CAM Polyclonal antibody

L1CAM Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32885-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

L1cam, N-CAM-L1, CAML1, CD171, N CAM L1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Positive IHC detected inmouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32885-1-AP targets L1CAM in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen L1CAM fusion protein Eg3022 相同性解析による交差性が予測される生物種
Full Name L1 cell adhesion molecule
Calculated molecular weight141 kDa
Observed molecular weight200-220 kDa, 140 kDa
GenBank accession numberN/A
Gene Symbol L1cam
Gene ID (NCBI) 16728
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP11627
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503). It has been reported that L1CAM can be proteolytically cleaved into a soluble fragment of 140 kDa and an intracellular fragment of 80-85 kDa (PMID: 30842511; 34380733).

Protocols

Product Specific Protocols
WB protocol for L1CAM antibody 32885-1-APDownload protocol
IHC protocol for L1CAM antibody 32885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols