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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using L1CAM/CD171 Polyclonal antibody (31534-1-AP)

    WB analysis of HeLa using 31534-1-AP

    WB result of L1CAM/CD171 antibody (31534-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-L1CAM/CD171 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using L1CAM Polyclonal antibody (31534-1-AP)

    WB analysis using 31534-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31534-1-AP (L1CAM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using L1CAM/CD171 Polyclonal antibody (31534-1-AP)

    IHC staining of mouse kidney using 31534-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31534-1-AP (L1CAM/CD171 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using L1CAM/CD171 Polyclonal antibody (31534-1-AP)

    IHC staining of mouse kidney using 31534-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31534-1-AP (L1CAM/CD171 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using L1CAM Polyclonal antibody (31534-1-AP)

    IF Staining of HeLa using 31534-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using L1CAM antibody (31534-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

L1CAM/CD171 Polyclonal antibody

L1CAM/CD171 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31534-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

L1CAM, CAML1, CD171, HSAS, HSAS1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, rat brain tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31534-1-AP targets L1CAM/CD171 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Eg0209

Product name: Recombinant Human L1CAM protein (Myc Tag, His Tag)

Source: mammalian cells-derived, pHZ-KIsec

Tag: Myc & 6*His

Domain: 20-1120 aa of BC126229

Sequence: IQIPEEYEGHHVMEPPVITEQSPRRLVVFPTDDISLKCEASGKPEVQFRWTRDGVHFKPKEELGVTVYQSPHSGSFTITGNNSNFAQRFQGIYRCFASNKLGTAMSHEIRLMAEGAPKWPKETVKPVEVEEGESVVLPCNPPPSAEPLRIYWMNSKILHIKQDERVTMGQNGNLYFANVLTSDNHSDYICHAHFPGTRTIIQKEPIDLRVKATNSMIDRKPRLLFPTNSSSHLVALQGQPLVLECIAEGFPTPTIKWLRPSGPMPADRVTYQNHNKTLQLLKVGEEDDGEYRCLAENSLGSARHAYYVTVEAAPYWLHKPQSHLYGPGETARLDCQVQGRPQPEVTWRINGIPVEELAKDQKYRIQRGALILSNVQPSDTMVTQCEARNRHGLLLANAYIYVVQLPAKILTADNQTYMAVQGSTAYLLCKAFGAPVPSVQWLDEDGTTVLQDERFFPYANGTLGIRDLQANDTGRYFCLAANDQNNVTIMANLKVKDATQITQGPRSTIEKKGSRVTFTCQASFDPSLQPSITWRGDGRDLQELGDSDKYFIEDGRLVIHSLDYSDQGNYSCVASTELDVVESRAQLLVVGSPGPVPRLVLSDLHLLTQSQVRVSWSPAEDHNAPIEKYDIEFEDKEMAPEKWYSLGKVPGNQTSTTLKLSPYVHYTFRVTAINKYGPGEPSPVSETVVTPEAAPEKNPVDVKGEGNETTNMVITWKPLRWMDWNAPQVQYRVQWRPQGTRGPWQEQIVSDPFLVVSNTSTFVPYEIKVQAVNSQGKGPEPQVTIGYSGEDYPQAIPELEGIEILNSSAVLVKWRPVDLAQVKGHLRGYNVTYWREGSQRKHSKRHIHKDHVVVPANTTSVILSGLRPYSSYHLEVQAFNGRGSGPASEFTFSTPEGVPGHPEALHLECQSNTSLLLRWQPPLSHNGVLTGYVLSYHPLDEGGKGQLSFNLRDPELRTHNLTDLSPHLRYRFQLQATTKEGPGEAIVREGGTMALSGISDFGNISATAGENYSVVSWVPKEGQCNFRFHILFKALGEEKGGASLSPQYVSYNQSSYTQWDLQPDTDYEIHLFKERMFRHQMAVKTNGTGRVRLPPAGFATE

相同性解析による交差性が予測される生物種
Full Name L1 cell adhesion molecule
Calculated molecular weight 1257 aa, 140 kDa
Observed molecular weight200-250 kDa
GenBank accession numberBC126229
Gene Symbol L1CAM
Gene ID (NCBI) 3897
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDP32004
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503). It has been reported that L1CAM can be proteolytically cleaved into a soluble fragment of 140 kDa and an intracellular fragment of 80-85 kDa (PMID: 30842511; 34380733). This antibody was raised against 20-1120 aa of human L1CAM.

Protocols

Product Specific Protocols
IF protocol for L1CAM/CD171 antibody 31534-1-APDownload protocol
IHC protocol for L1CAM/CD171 antibody 31534-1-APDownload protocol
WB protocol for L1CAM/CD171 antibody 31534-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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