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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using L1CAM Polyclonal antibody (31534-1-AP)

    WB analysis using 31534-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31534-1-AP (L1CAM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using L1CAM Polyclonal antibody (31534-1-AP)

    IF Staining of HeLa using 31534-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using L1CAM antibody (31534-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

L1CAM Polyclonal antibody

L1CAM Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31534-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

CAML1, CD171, HSAS, HSAS1, L1 cell adhesion molecule

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31534-1-AP targets L1CAM in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen L1CAM fusion protein Eg0209 相同性解析による交差性が予測される生物種
Full Name L1 cell adhesion molecule
Calculated molecular weight 1257 aa, 140 kDa
Observed molecular weight200-220 kDa
GenBank accession numberBC126229
Gene Symbol L1CAM
Gene ID (NCBI) 3897
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP32004
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503). It has been reported that L1CAM can be proteolytically cleaved into a soluble fragment of 140 kDa and an intracellular fragment of 80-85 kDa (PMID: 30842511; 34380733). This antibody was raised against 20-1120 aa of human L1CAM.

Protocols

Product Specific Protocols
WB protocol for L1CAM antibody 31534-1-APDownload protocol
IF protocol for L1CAM antibody 31534-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols