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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using Klk1 Recombinant monoclonal antibody (86143-1-RR)

    WB analysis of mouse pancreas using 86143-1-RR (same clone as 86143-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 86143-1-RR (Klk1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86143-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Klk1 Recombinant monoclonal antibody (86143-1-RR)

    IHC staining of mouse pancreas using 86143-1-RR (same clone as 86143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 86143-1-RR (Klk1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86143-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Klk1 Recombinant monoclonal antibody (86143-1-RR)

    IHC staining of mouse pancreas using 86143-1-RR (same clone as 86143-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 86143-1-RR (Klk1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86143-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Klk1 Recombinant monoclonal antibody (86143-1-RR)

    IF Staining of mouse pancreas using 86143-1-RR (same clone as 86143-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Klk1 antibody (86143-1-RR, Clone: 250034D2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86143-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using Klk1 Recombinant monoclonal antibody (86143-1-RR)

    IF Staining of mouse pancreas using 86143-1-RR (same clone as 86143-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Klk1 antibody (86143-1-RR, Clone: 250034D2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86143-1-PBS in a different storage buffer formulation.

Mouse Klk1 Recombinant monoclonal antibody, PBS Only

Klk1 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 86143-1-PBS
Clone No.250034D2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Kallikrein 1, EC:3.4.21.35, Glandular kallikrein K1, KAL-B, Kallikrein-1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86143-1-PBS targets Klk1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg2996

Product name: recombinant mouse Klk1 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 19-261 aa of NM_001320331.1

Sequence: PPVQSRIVGGFNCEKNSQPWQVAVYRFTKYQCGGILLNANWVLTAAHCHNDKYQVWLGKNNFLEDEPSAQHRLVSKAIPHPDFNMSLLNEHTPQPEDDYSNDLMLLRLKKPADITDVVKPIDLPTEEPKLGSTCLASGWGSITPVKYEYPDELQCVNLKLLPNEDCAKAHIEKVTDDMLCAGDMDGGKDTCAGDSGGPLICDGVLQGITSWGPSPCGKPNVPGIYTRVLNFNTWIRETMAEND

相同性解析による交差性が予測される生物種
Full Name kallikrein 1
Calculated molecular weight29 kDa
Observed molecular weight28-34 kDa
GenBank accession numberNM_001320331.1
Gene Symbol Klk1
Gene ID (NCBI) 16612
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP15947
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Mouse KLK1 (Kallikrein-Related Peptidase 1) is a serine protease belonging to the kallikrein family with important physiological functions. KLK1 can cleave the Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Bradykinin plays an important role in regulating vasodilation, lowering blood pressure, smooth muscle relaxation and contraction, pain induction and inflammatory response.

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