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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Kininogen-1 Recombinant antibody (84175-5-RR)

    WB analysis of human plasma using 84175-5-RR

    Human plasma was subjected to SDS PAGE followed by western blot with 84175-5-RR (Kininogen-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HT-29 cells using Kininogen-1 Recombinant antibody (84175-5-RR)

    FC experiment of HT-29 using 84175-5-RR

    1x10^6 HT-29 cells were intracellularly stained with 0.25 ug Kininogen-1 Recombinant antibody (84175-5-RR, Clone:241397C11) (red), or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84175-5-RR

    Affinity and Kinetic Characterization of 84175-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84175-5-RR against Human Kininogen-1 were performed. The affinity constant is 0.182 nM.

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Kininogen 1 Recombinant antibody

Kininogen 1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84175-5-RR
Clone No.241397C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

KNG1, 241397C11, Alpha-2-thiol proteinase inhibitor, BDK, Bradykinin

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman plasma
Positive FC (Intra) detected inHT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84175-5-RR targets Kininogen 1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name kininogen 1
Calculated molecular weight72kDa
Observed molecular weight 48 kDa
GenBank accession numberNM_001102416.3
Gene Symbol Kininogen 1
Gene ID (NCBI) 3827
RRIDAB_3671732
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01042-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This KNG1 gene contains 10 exons and encodes two proteins through alternative splicing: high molecular weight kininogen (HMWK) (72kDa) and low molecular weight kininogen (LMWK) (48kDa) (PMID: 37442062). HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.

Protocols

Product Specific Protocols
WB protocol for Kininogen 1 antibody 84175-5-RRDownload protocol
FC protocol for Kininogen 1 antibody 84175-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols