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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using Kininogen 1 Monoclonal antibody (66123-1-Ig)

    WB analysis of human heart using 66123-1-Ig (same clone as 66123-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using Kininogen 1 Monoclonal antibody (66123-1-Ig)

    WB analysis of human placenta using 66123-1-Ig (same clone as 66123-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human plasma using Kininogen 1 Monoclonal antibody (66123-1-Ig)

    WB analysis of human plasma using 66123-1-Ig (same clone as 66123-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Kininogen 1 Monoclonal antibody (66123-1-Ig)

    IHC staining of human breast cancer using 66123-1-Ig (same clone as 66123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Kininogen 1 Monoclonal antibody (66123-1-Ig)

    IHC staining of human breast cancer using 66123-1-Ig (same clone as 66123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Kininogen 1 Monoclonal antibody (66123-1-Ig)

    IHC staining of human ovary tumor using 66123-1-Ig (same clone as 66123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Kininogen 1 Monoclonal antibody (66123-1-Ig)

    IHC staining of human ovary tumor using 66123-1-Ig (same clone as 66123-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66123-1-Ig (Kininogen 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Kininogen 1 Monoclonal antibody (66123-1-Ig)

    IF Staining of HeLa using 66123-1-Ig (same clone as 66123-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Kininogen 1 antibody (66123-1-Ig, Clone: 10E8D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66123-1-PBS in a different storage buffer formulation.

Kininogen 1 Monoclonal antibody, PBS Only

Kininogen 1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66123-1-PBS
Clone No.10E8D11

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

BDK, Fitzgerald factor, HMWK, Ile Ser Bradykinin Bradykinin, Kallidin I Lysyl bradykinin, kininogen 1, KNG, KNG1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66123-1-PBS targets Kininogen 1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag19233

Product name: Recombinant human KNG1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 20-374 aa of BC060039

Sequence: ESQSEEIDCNDKDLFKAVDAALKKYNSQNQSNNQFVLYRITEATKTVGSDTFYSFKYEIKEGDCPVQSGKTWQDCEYKDAAKAATGECTATVGKRSSTKFSVATQTCQITPAEGPVVTAQYDCLGCVHPISTQSPDLEPILRHGIQYFNNNTQHSSLFMLNEVKRAQRQVVAGLNFRMTYSIVQTNCSKENFLFLTPDCKSLWNGDTGECTDNAYIDIQLRIASFSQNCDIYPGKDFVQPPTKICVGCPRDIPTNSPELEETLTHTITKLNAENNATFYFKIDNVKKARVQVVAGKKYFIDFVARETTCSKESNEELTESCETKKLGQSLDCNAEVYVVPWEKKIYPTVNCQPLG

相同性解析による交差性が予測される生物種
Full Name kininogen 1
Calculated molecular weight 644 aa, 72 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC060039
Gene Symbol Kininogen 1
Gene ID (NCBI) 3827
RRIDAB_2881522
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP01042
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This gene is translated into High-molecular weight kininogen (HMWK) and low-molecular weight kininogen (LMWK) after alternative splicing. HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.

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