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Ki-67 Recombinant monoclonal antibody

Ki-67 Uni-rAb® Recombinant Antibody for WB, IHC, IHC-Autostainer, IF/ICC, IF-P, ELISA
Cat No. 84192-4-RR
Clone No.241499E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IHC-Autostainer, IF/ICC, IF-P, ELISA

KI67, MKI67, 241499E7, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, MCF-7 cells, Ramos cells
Positive IHC-Autostainer detected inhuman tonsil tissue, human tonsillitis tissue, human stomach cancer tissue, human breast cancer tissue, human appendicitis tissue, human liver cancer tissue
Positive IHC detected inhuman tonsillitis tissue, Cholangiocarcinoma tissue, human Breast cancer tissue, human osteosarcoma tissue, human pancreas cancer tissue, Human Spleen tissue, human liver cancer tissue, human skin cancer tissue, human liver tissue, human stomach cancer tissue, human appendicitis tissue, human prostate cancer tissue, human malignant melanoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung cancer tissue, human thyroid cancer tissue
Positive IF/ICC detected inHeLa cells, MCF-7 cells, U2OS cells, HepG2 cells, A431 cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)-AUTOSTAINERIHC-AUTOSTAINER : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84192-4-RR targets Ki-67 in WB, IHC, IHC-Autostainer, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name antigen identified by monoclonal antibody Ki-67
Calculated molecular weight 359 kDa
Observed molecular weight359 kDa
GenBank accession numberNM_002417
Gene Symbol KI67
Gene ID (NCBI) 4288
RRIDAB_3671744
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP46013
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

Protocols

Product Specific Protocols
IF protocol for Ki-67 antibody 84192-4-RRDownload protocol
IHC protocol for Ki-67 antibody 84192-4-RRDownload protocol
WB protocol for Ki-67 antibody 84192-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Time-resolved multiomics profiling reveals chromatin O-GlcNAc modification promotes senescence-associated transcriptional program.

Authors - Nana Zhang
humanIHC

Acta Pharm Sin B

Thermal proteome profiling (TPP) reveals NAMPT as the anti-glioma target of phenanthroindolizidine alkaloid PF403

Authors - Fangfei Li
IHC

Research (Wash D C)

Hypoxic Reprogramming of ACOX1-Driven HSP90AB1 Crotonylation Stabilizes Thioredoxin to Orchestrate Redox Homeostasis in Oral Squamous Cell Carcinoma.

Authors - Xiteng Yin
mouse,humanWB

Cell Mol Biol Lett

The promoting roles of GLP1R and GIPR in stemness maintenance and multiple lineage-specific differentiation of PDLSCs.

Authors - Yifen Shen
humanIHC

Cancer Lett

Apigenin Inhibits Recurrent Bladder Cancer Progression by Targeting VEGF-β

Authors - Zhen-Duo Shi
humanWB,IHC

J Transl Med

Epstein-Barr virus-coded miR-BART19-3p promotes proliferation of EBV-associated gastric cancer by inhibiting GADD45B.

Authors - Jing Yang
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