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Tested Applications
| Positive WB detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31197-1-AP targets Keap1 in WB, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Keap1 fusion protein Ag35035 相同性解析による交差性が予測される生物種 |
| Full Name | kelch-like ECH-associated protein 1 |
| Calculated molecular weight | 70 kDa |
| Observed molecular weight | 60-64 kDa |
| GenBank accession number | NM_001110305 |
| Gene Symbol | Keap1 |
| Gene ID (NCBI) | 50868 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9Z2X8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KEAP1 (Kelch-like ECH-associated protein 1) is a critical regulatory protein that plays a pivotal role in the cellular response to oxidative stress. It is best known for its function as a negative regulator of the Nrf2 (NF-E2-related factor 2) pathway, which is essential for maintaining cellular redox homeostasis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Keap1 antibody 31197-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |