Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
86555-3-PBS targets KYNU in WB, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag2365 Product name: Recombinant human KYNU protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-307 aa of BC000879 Sequence: MEPSSLELPADTVQRIAAELKCHPTDERVALHLDEEDKLRHFRECFYIPKIQDLPPVDLSLVNKDENAIYFLGNSLGLQPKMVKTYLEEELDKWAKIAAYGHEVGKRPWITGDESIVGLMKDIVGANEKEIALMNALTVNLHLLMLSFFKPTPKRYKILLEAKAFPSDHYAIESQLQLHGLNIEESMRMIKPREGEETLRIEDILEVIEKEGDSIAVILFSGVHFYTGQHFNIPAITKAGQAKGCYVGFDLAHAVGNVELYLHDWGVDFACWCSYKYLNAGAGGIAGAFIHEKHAHTIKPARSEFFN 相同性解析による交差性が予測される生物種 |
| Full Name | kynureninase (L-kynurenine hydrolase) |
| Calculated molecular weight | 465aa,52 kDa; 307aa,35 kDa |
| Observed molecular weight | 45-52 kDa |
| GenBank accession number | BC000879 |
| Gene Symbol | KYNU |
| Gene ID (NCBI) | 8942 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16719 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
KYNU(kynureninase) is also named as L-kynurenine hydrolase and belongs to the kynureninase family. It is a pyridoxal-5'-phosphate-(pyridoxal-P)-depe11dent enzyme that catalyses the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively(PMID:8706755). In SDS-PAGE, it can detect a 48 kDa band lower than the preidicted molecular weight of 52 kDa due to limited proteolysis of the enzyme which might occur during handling of the protein(PMID:8706755).